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Literature summary for 6.1.1.3 extracted from

  • Nagaoka, Y.; Ishikura, K.; Kuno, A.; Hasegawa, T.
    Evolution and tRNA recognition of threonyl-tRNA synthetase from an extreme thermophilic archaeon, Aeropyrum pernix K1 (2003), Viva Orig., 31, 62-70.
No PubMed abstract available
Search for more literature for 6.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence analysis Aeropyrum pernix

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ mediates amino acid discrimination, binding model Aeropyrum pernix

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-threonine + tRNAThr Haloferax volcanii
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
ATP + L-threonine + tRNAThr Aeropyrum pernix
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YDW0 isozyme ThrRS-2, K1, extreme thermophilic archaeon
-
Aeropyrum pernix Q9YFY3 isozyme ThrRS-1, K1, extreme thermophilic archaeon
-
Haloferax volcanii
-
 extreme halophilic archaeon
-

Purification (Commentary)

Purification (Comment) Organism
partially Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-threonine + tRNAThr
-
 Haloferax volcanii AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
ATP + L-threonine + tRNAThr
-
 Aeropyrum pernix AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
ATP + L-threonine + tRNAThr secondary structure of the tRNAThr, enzyme utilizes substrates from archaeon with discriminator base U73 and Escherichia coli with discriminator base A73 Aeropyrum pernix AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
ATP + L-threonine + tRNAThr secondary structure of the tRNAThr, specific for archaeal tRNAThr with discriminator base U73, no activity with one of Escherichia coli with discriminator base A73 Haloferax volcanii AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Subunits

Subunits Comment Organism
More structure schematic model Aeropyrum pernix

Synonyms

Synonyms Comment Organism
Synthetase, threonyl-transfer ribonucleate
-
 Haloferax volcanii
Synthetase, threonyl-transfer ribonucleate
-
 Aeropyrum pernix
Threonine translase
-
 Haloferax volcanii
Threonine translase
-
 Aeropyrum pernix
Threonine--tRNA ligase
-
 Haloferax volcanii
Threonine--tRNA ligase
-
 Aeropyrum pernix
Threonine-transfer ribonucleate synthetase
-
 Haloferax volcanii
Threonine-transfer ribonucleate synthetase
-
 Aeropyrum pernix
Threonyl-ribonucleic synthetase
-
 Haloferax volcanii
Threonyl-ribonucleic synthetase
-
 Aeropyrum pernix
Threonyl-transfer ribonucleate synthetase
-
 Haloferax volcanii
Threonyl-transfer ribonucleate synthetase
-
 Aeropyrum pernix
Threonyl-transfer ribonucleic acid synthetase
-
 Haloferax volcanii
Threonyl-transfer ribonucleic acid synthetase
-
 Aeropyrum pernix
Threonyl-transfer RNA synthetase
-
 Haloferax volcanii
Threonyl-transfer RNA synthetase
-
 Aeropyrum pernix
Threonyl-tRNA synthetase
-
 Haloferax volcanii
Threonyl-tRNA synthetase
-
 Aeropyrum pernix
ThrRS
-
 Haloferax volcanii
ThrRS
-
 Aeropyrum pernix
TRS
-
 Haloferax volcanii
TRS
-
 Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aeropyrum pernix
50
-
 assay at Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Haloferax volcanii
7.5
-
 assay at Aeropyrum pernix

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Haloferax volcanii
ATP
-
 Aeropyrum pernix