Literature summary for 6.1.1.4 extracted from

Fukunaga, R.; Ishitani, R.; Nureki, O.; Yokoyama, S.
Crystallization of leucyl-tRNA synthetase complexed with tRNALeu from the archaeon Pyrococcus horikoshii (2004), Acta Crystallogr. Sect. F, 61, 30-32.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment)	Organism
cocrystallizations with each of the tRNALeu isoacceptors are attempted. Cocrystals are obtained by the hanging-drop vapour-diffusion method, but only when the tRNALeu isoacceptor with the anticodon CAA is used. Electrophoretic analyses reveals that the crystals contain both leucyl-tRNA synthetase and tRNALeu, suggesting that they are LeuRS-tRNALeu complex crystals. A data set diffracting to 3.3 A resolution is collected from a single crystal at 100 K. The crystal belongs to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 118.18, b = 120.55, c = 231.13 A	Pyrococcus horikoshii

Crystallization (Comment)

Organism

cocrystallizations with each of the tRNALeu isoacceptors are attempted. Cocrystals are obtained by the hanging-drop vapour-diffusion method, but only when the tRNALeu isoacceptor with the anticodon CAA is used. Electrophoretic analyses reveals that the crystals contain both leucyl-tRNA synthetase and tRNALeu, suggesting that they are LeuRS-tRNALeu complex crystals. A data set diffracting to 3.3 A resolution is collected from a single crystal at 100 K. The crystal belongs to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 118.18, b = 120.55, c = 231.13 A

Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	-	-	-

Synonyms

Synonyms	Comment	Organism
Leucyl-tRNA synthetase	-	Pyrococcus horikoshii
LeuRS	-	Pyrococcus horikoshii

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)