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Literature summary for 6.1.1.5 extracted from

  • Pope, A.J.; Lapointe, J.; Mensah, L.; Benson, N.; Brown, M.J.; Moore, K.J.
    Characterization of isoleucyl-tRNA synthetase from Staphylococcus aureus. I: Kinetic mechanism of the substrate activation reaction studied by transient and steady-state techniques (1998), J. Biol. Chem., 273, 31680-31690.
    View publication on PubMed
Search for more literature for 6.1.1.5

Inhibitors

Inhibitors Comment Organism Structure
Ile-ol-AMP
-
 Staphylococcus aureus
isoleucinyl adenylate i.e. Ile-ol-AMP, nonhydrolyzable reaction intermediate analogue, competitive with respect to both ATP and Ile Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information reaction kinetics Staphylococcus aureus
0.0001
-
 tRNAIle below, tRNAIle aminoacylation reaction, pH 7.9, 22°C Staphylococcus aureus
0.005
-
 L-isoleucine tRNAIle aminoacylation reaction, pH 7.9, 22°C Staphylococcus aureus
0.01
-
 L-isoleucine ATP-diphosphate exchange reaction, pH 7.9, 22°C Staphylococcus aureus
0.24
-
 ATP tRNAIle aminoacylation reaction, pH 7.9, 22°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-isoleucine + tRNAIle Staphylococcus aureus
-
 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 r

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-isoleucine + tRNAIle = AMP + diphosphate + L-isoleucyl-tRNAIle reaction kinetics and mechanism Staphylococcus aureus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-isoleucine + tRNAIle
-
 Staphylococcus aureus AMP + diphosphate + L-isoleucyl-tRNAIle
-
 r
ATP + L-isoleucine + tRNAIle formation of a enzyme-bound aminoacyl adenylate intermediate Staphylococcus aureus AMP + diphosphate + L-isoleucyl-tRNAIle
-
 r
additional information enzyme also performs the reversible ATP-diphosphate exchange reaction Staphylococcus aureus ?
-
 ?

Synonyms

Synonyms Comment Organism
IleRS
-
 Staphylococcus aureus
IRS
-
 Staphylococcus aureus
Isoleucine translase
-
 Staphylococcus aureus
Isoleucine--tRNA ligase
-
 Staphylococcus aureus
Isoleucine-transfer RNA ligase
-
 Staphylococcus aureus
Isoleucine-tRNA synthetase
-
 Staphylococcus aureus
Isoleucyl-transfer ribonucleate synthetase
-
 Staphylococcus aureus
Isoleucyl-transfer RNA synthetase
-
 Staphylococcus aureus
Isoleucyl-tRNA synthetase
-
 Staphylococcus aureus
Mupirocin resistance protein
-
 Staphylococcus aureus
Synthetase, isoleucyl-transfer ribonucleate
-
 Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 L-isoleucine tRNAIle aminoacylation reaction, pH 7.9, 22°C Staphylococcus aureus
18
-
 L-isoleucine ATP-diphosphate exchange reaction, pH 7.9, 22°C Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
ATP binds more tightly to the enzyme-Ile complex than to the free enzyme Staphylococcus aureus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00003
-
 Ile-ol-AMP pH 7.9, 22°C Staphylococcus aureus