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Literature summary for 6.1.1.5 extracted from

  • Pope, A.J.; Moore, K.J.; McVey, M.; Mensah, L.; Benson, N.; Osbourne, N.; Broom, N.; Brown, M.J.; O'Hanlon, P.
    Characterization of isoleucyl-tRNA synthetase from Staphylococcus aureus. II. Mechanism of inhibition by reaction intermediate and pseudomonic acid analogues studied using transient and steady-state kinetics (1998), J. Biol. Chem., 273, 31691-31701.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ethyl monate-A
-
Staphylococcus aureus
Ile-NHSO2-AMP non-hydrolyzable reaction intermediate analogue, slow-tight binding, competitive and inhibition mechanism, reversible Staphylococcus aureus
isoleucinyl-adenylate i.e. Ile-ol-AMP, non-hydrolyzable reaction intermediate analogue, slow-tight binding, competitive and inhibition mechanism, reversible Staphylococcus aureus
pseudomonic acid forms a non-hydrolyzable reaction intermediate analogue, competitive inhibition Staphylococcus aureus
SB-205952 a semisynthetic analogue of monic acid, possesing a nitrofuryl chromophore Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-isoleucine + tRNAIle Staphylococcus aureus
-
AMP + diphosphate + L-isoleucyl-tRNAIle
-
r

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-isoleucine + tRNAIle
-
Staphylococcus aureus AMP + diphosphate + L-isoleucyl-tRNAIle
-
r
additional information enzyme also performs the reversible ATP-diphosphate exchange reaction Staphylococcus aureus ?
-
?

Synonyms

Synonyms Comment Organism
IleRS
-
Staphylococcus aureus
IRS
-
Staphylococcus aureus
Isoleucine translase
-
Staphylococcus aureus
Isoleucine--tRNA ligase
-
Staphylococcus aureus
Isoleucine-transfer RNA ligase
-
Staphylococcus aureus
Isoleucine-tRNA synthetase
-
Staphylococcus aureus
Isoleucyl-transfer ribonucleate synthetase
-
Staphylococcus aureus
Isoleucyl-transfer RNA synthetase
-
Staphylococcus aureus
Isoleucyl-tRNA synthetase
-
Staphylococcus aureus
Mupirocin resistance protein
-
Staphylococcus aureus
Synthetase, isoleucyl-transfer ribonucleate
-
Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
assay at Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Staphylococcus aureus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Staphylococcus aureus
0.000003
-
Ile-NHSO2-AMP pH 7.9, 22°C Staphylococcus aureus
0.00002
-
pseudomonic acid pH 7.9, 22°C Staphylococcus aureus
0.00004
-
ethyl monate-A pH 7.9, 22°C Staphylococcus aureus
0.000045
-
SB-205952 pH 7.9, 22°C Staphylococcus aureus
0.00009
-
isoleucinyl-adenylate pH 7.9, 22°C Staphylococcus aureus