Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chymotrypsin | proteolytic inactivation patterns, bound Ile-AMP or inhibitors isoleucinol adenylate and pseudomonic acid protect, 50fold higher concentration is needed for digestion of Ile-AMP-enzyme complex than for the free enzyme at 37°C | Staphylococcus aureus | |
isoleucinol adenylate | determination of binding structures, bound inhibitor protects against proteolytic inactivation by trypsin or chymotrypsin and specifically alters the proteolytic cleavage pattern | Staphylococcus aureus | |
pseudomonic acid | competitive, determination of binding structures, bound inhibitor protects against proteolytic inactivation by trypsin or chymotrypsin and specifically alters the proteolytic cleavage pattern | Staphylococcus aureus | |
SB-205952 | a semisynthetic analogue of monic acid | Staphylococcus aureus | |
Trypsin | proteolytic inactivation patterns, bound Ile-AMP or inhibitors isoleucinol adenylate and pseudomonic acid protect, 50fold higher concentration is needed for digestion of Ile-AMP-enzyme complex than for the free enzyme at 37°C | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
tRNAIle | below, pH 7.9, 22°C | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, MgATP2- | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | Staphylococcus aureus | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
purified recombinant enzyme expressed in Escherichia coli strain DH1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | - |
Staphylococcus aureus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
r | |
ATP + L-isoleucine + tRNAIle | reaction intermediate is the Ile-AMP-enzyme complex | Staphylococcus aureus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
r | |
additional information | enzyme also performs the reversible ATP-diphosphate exchange reaction | Staphylococcus aureus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IleRS | - |
Staphylococcus aureus |
IRS | - |
Staphylococcus aureus |
Isoleucine translase | - |
Staphylococcus aureus |
Isoleucine--tRNA ligase | - |
Staphylococcus aureus |
Isoleucine-transfer RNA ligase | - |
Staphylococcus aureus |
Isoleucine-tRNA synthetase | - |
Staphylococcus aureus |
Isoleucyl-transfer ribonucleate synthetase | - |
Staphylococcus aureus |
Isoleucyl-transfer RNA synthetase | - |
Staphylococcus aureus |
Isoleucyl-tRNA synthetase | - |
Staphylococcus aureus |
Mupirocin resistance protein | - |
Staphylococcus aureus |
Synthetase, isoleucyl-transfer ribonucleate | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on, MgATP2- | Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000001 | - |
pseudomonic acid | pH 7.9, 22°C, in complex with the enzyme and Ile, in analogy to the reaction intermediate | Staphylococcus aureus | |
0.00003 | - |
isoleucinol adenylate | pH 7.9, 22°C, in complex with the enzyme and Ile, in analogy to the reaction intermediate | Staphylococcus aureus |