Cloned (Comment) | Organism |
---|---|
expression in strain BL21(DE3) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | - |
Borreliella burgdorferi | |
5'-O-[N-(L-lysyl)sulfamoyl]adenosine | - |
Escherichia coli | |
AEC | competitive versus L-lysine | Escherichia coli | |
cadaverine | - |
Escherichia coli | |
D-Lysine | competitive versus L-lysine | Escherichia coli | |
gamma-aminobutyric acid | - |
Borreliella burgdorferi | |
L-arginine | - |
Borreliella burgdorferi | |
lysinamide | competitive versus L-lysine | Escherichia coli | |
lysine ethyl ester | competitive versus L-lysine | Escherichia coli | |
lysine methyl ester | competitive versus L-lysine | Escherichia coli | |
additional information | no inhibition by lysinamide in vivo | Borreliella burgdorferi | |
ornithine | competitive versus L-lysine | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + tRNALys | Escherichia coli | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | Borreliella burgdorferi | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Borreliella burgdorferi | - |
purified enzyme from Bacillus subtilis strain 157.1, class I enzyme | - |
Escherichia coli | - |
gene lysS, class II enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant from strain BL21(DE3) | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys | active site structure | Escherichia coli | |
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys | active site structure | Borreliella burgdorferi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-aminobutanoate + tRNALys | - |
Escherichia coli | AMP + 4-aminobutyryl-tRNALys + diphosphate | - |
? | |
ATP + D-lysine + tRNALys | - |
Escherichia coli | AMP + D-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-arginine + tRNALys | low activity | Escherichia coli | AMP + L-arginyl-tRNALys + diphosphate | - |
? | |
ATP + L-glutamate + tRNALys | low activity | Escherichia coli | AMP + L-glutamyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysinamide + tRNALys | low activity | Escherichia coli | AMP + L-aminolysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | - |
Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | - |
Borreliella burgdorferi | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | 2-step reaction, the first step comprises the activation of the amino acid to form an enzyme-bound aminoacyl adenylate, the second step involves binding of this complex by tRNA, whose 3'-end is esterified with the aminoacyl-moiety followed by release of the resulting aminoacyl-tRNA, aminoacylation can be performed in absence of the tRNA | Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine + tRNALys | 2-step reaction, the first step comprises the activation of the amino acid to form an enzyme-bound aminoacyl adenylate, the second step involves binding of this complex by tRNA, whose 3'-end is esterified with the aminoacyl-moiety followed by release of the resulting aminoacyl-tRNA, aminoacylation cannot be performed in absence of the tRNA | Borreliella burgdorferi | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine ethyl ester + tRNALys | - |
Escherichia coli | AMP + ethyl-L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine ethyl ester + tRNALys | - |
Borreliella burgdorferi | AMP + ethyl-L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine hydroxamate + tRNALys | - |
Escherichia coli | AMP + L-lysine hydroxamoyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine methyl ester + tRNALys | - |
Escherichia coli | AMP + methyl-L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + L-lysine methyl ester + tRNALys | - |
Borreliella burgdorferi | AMP + methyl-L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + ornithine + tRNALys | - |
Escherichia coli | AMP + ornithyl-tRNALys + diphosphate | - |
? | |
ATP + ornithine + tRNALys | low activity | Borreliella burgdorferi | AMP + ornithyl-tRNALys + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-Lysine-transfer RNA ligase | - |
Escherichia coli |
L-Lysine-transfer RNA ligase | - |
Borreliella burgdorferi |
Lysine translase | - |
Escherichia coli |
Lysine translase | - |
Borreliella burgdorferi |
Lysine--tRNA ligase | - |
Escherichia coli |
Lysine--tRNA ligase | - |
Borreliella burgdorferi |
Lysine-tRNA synthetase | - |
Escherichia coli |
Lysine-tRNA synthetase | - |
Borreliella burgdorferi |
LysRS | - |
Escherichia coli |
LysRS | - |
Borreliella burgdorferi |
LysRS1 | class I enzyme | Borreliella burgdorferi |
LysRS2 | class II enzyme | Escherichia coli |
Lysyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Lysyl-transfer ribonucleate synthetase | - |
Borreliella burgdorferi |
Lysyl-transfer RNA synthetase | - |
Escherichia coli |
Lysyl-transfer RNA synthetase | - |
Borreliella burgdorferi |
Lysyl-tRNA synthetase | - |
Escherichia coli |
Lysyl-tRNA synthetase | - |
Borreliella burgdorferi |
Synthetase, lysyl-transfer ribonucleate | - |
Escherichia coli |
Synthetase, lysyl-transfer ribonucleate | - |
Borreliella burgdorferi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Borreliella burgdorferi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
L-arginine | pH 7.2, 37°C | Borreliella burgdorferi | |
12 | - |
D-Lysine | pH 7.2, 37°C | Escherichia coli |