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Literature summary for 6.2.1.1 extracted from

  • Martinez-Blanco, H.; Reglero, A.; Fenandez-Valverde, M.; Ferrero, M.A.; Moreno, M.A.; Penalva, M.A.; Luengo, J.M.
    Isolation and characterization of the acetyl-CoA synthetase from Penicillium chrysogenum (1992), J. Biol. Chem., 267, 5474-5481.
    View publication on PubMed
Search for more literature for 6.2.1.1

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Penicillium chrysogenum
DTT stimulates Penicillium chrysogenum
GSH stimulates Penicillium chrysogenum

General Stability

General Stability Organism
particularly sensitive to repeated freezing Penicillium chrysogenum

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Penicillium chrysogenum
Hg2+
-
 Penicillium chrysogenum
NEM
-
 Penicillium chrysogenum
Nonidet P40 weak Penicillium chrysogenum
p-chloromercuribenzoate
-
 Penicillium chrysogenum
Tween 100
-
 Penicillium chrysogenum
Tween 20
-
 Penicillium chrysogenum
Tween 80
-
 Penicillium chrysogenum
Zn2+
-
 Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 CoA
-
 Penicillium chrysogenum
6.8
-
 acetate
-
 Penicillium chrysogenum
17
-
 ATP
-
 Penicillium chrysogenum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Penicillium chrysogenum
Mn2+ can replace Mg2+ in activation Penicillium chrysogenum
Mn2+ 64% of the activation relative to Mg2+ Penicillium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 2 * 70000, SDS-PAGE Penicillium chrysogenum
139000
-
 gel filtration Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus nidulans the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins ?
-
 ?
additional information Penicillium chrysogenum the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-
Penicillium chrysogenum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium chrysogenum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Penicillium chrysogenum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
-
 Penicillium chrysogenum

Storage Stability

Storage Stability Organism
-20°C, cannot be stored for more than 3 days without a considerable loss of activity Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + acetate + CoA 37% of the activity relative to ATP Penicillium chrysogenum AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA
-
 Aspergillus nidulans AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA
-
 Penicillium chrysogenum AMP + diphosphate + acetyl-CoA
-
 ?
ATP + butyrate + CoA 20% of the activity relative to acetate Penicillium chrysogenum AMP + diphosphate + butyryl-CoA
-
 ?
ATP + pentanoate + CoA 6.7% of the activity relative to acetate Penicillium chrysogenum AMP + diphosphate + pentanoyl-CoA
-
 ?
ATP + propanoate + CoA 48% of the activity relative to acetate Penicillium chrysogenum AMP + diphosphate + propanoyl-CoA
-
 ?
CTP + acetate + CoA
-
 Penicillium chrysogenum CMP + diphosphate + acetyl-CoA
-
 ?
GTP + acetate + CoA
-
 Penicillium chrysogenum GMP + diphosphate + acetyl-CoA
-
 ?
additional information the enzyme can activate many other molecules to acyl-CoA derivatives: hexanoate, 3-hexenoate, heptanoate, octanoate, 3-octenoate, phenylacetate, 2-thiophene acetate, 3-thiophene acetate Penicillium chrysogenum ?
-
 ?
additional information the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins Aspergillus nidulans ?
-
 ?
additional information the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins Penicillium chrysogenum ?
-
 ?
UTP + acetate + CoA
-
 Penicillium chrysogenum UMP + diphosphate + acetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 70000, SDS-PAGE Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Penicillium chrysogenum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 45% of maximal activity, 50°C: about 30% of maximal activity Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Penicillium chrysogenum

pH Range

pH Minimum pH Maximum Comment Organism
6 9.7 6.0: about 45% of maximal activity, 9.7: about 70% of maximal activity Penicillium chrysogenum