Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 as His-tag fusion protein | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimeric proteins consisting of fragments of isoforms 4CL1 and 4CL2 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
4-coumarate | 4CL1, pH 7.8, room temperature | Arabidopsis thaliana | |
0.057 | - |
ferulate | 4CL1, pH 7.8, room temperature | Arabidopsis thaliana | |
0.22 | - |
4-coumarate | 4CL2, pH 7.8, room temperature | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Arabidopsis thaliana | plant phenylpropanoid metabolism | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isoforms 4CL1 and 4CL2 | - |
Purification (Comment) | Organism |
---|---|
using His-tag | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | plant phenylpropanoid metabolism | Arabidopsis thaliana | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + ferulate + CoA | only 4CL1, no substrate for 4CL2 | Arabidopsis thaliana | AMP + diphosphate + feruloyl-CoA | - |
? |