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Literature summary for 6.2.1.12 extracted from

  • Allina, S.M.; Pri-Hadash, A.; Theilmann, D.A.; Ellis, B.E.; Douglas, C.J.
    4-Coumarate:coenzyme A ligase in hybrid poplar. Properties of native enzymes, cDNA cloning, and analysis of recombinant enzymes (1998), Plant Physiol., 116, 743-754.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 cells using the baculovirus system Populus trichocarpa x Populus deltoides

General Stability

General Stability Organism
purified enzyme is very labile, no stabilizer found Populus trichocarpa x Populus deltoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 4-coumarate room temperature Populus trichocarpa x Populus deltoides
0.102
-
 ferulate room temperature Populus trichocarpa x Populus deltoides
1.048
-
 cinnamate room temperature Populus trichocarpa x Populus deltoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 SDS-PAGE Populus trichocarpa x Populus deltoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Populus trichocarpa x Populus deltoides plant phenylpropanoid metabolism AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Populus trichocarpa x Populus deltoides
-
 hybid poplar, clone H1
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant enzymes Populus trichocarpa x Populus deltoides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.013
-
 purified leaf enzyme, room temperature Populus trichocarpa x Populus deltoides
0.13
-
 purified cell culture enzyme, room temperature Populus trichocarpa x Populus deltoides
0.33
-
 purified xylem enzyme, room temperature Populus trichocarpa x Populus deltoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
 Populus trichocarpa x Populus deltoides AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA plant phenylpropanoid metabolism Populus trichocarpa x Populus deltoides AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + caffeate + CoA
-
 Populus trichocarpa x Populus deltoides AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + cinnamate + CoA roughly 30% of activity Populus trichocarpa x Populus deltoides AMP + diphosphate + cinnamoyl-CoA
-
 ?
ATP + ferulate + CoA roughly 60% of activity Populus trichocarpa x Populus deltoides AMP + diphosphate + feruloyl-CoA
-
 ?
additional information inactive against sinapic acid Populus trichocarpa x Populus deltoides ?
-
 ?