Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as His-tag fusion protein | Pinus taeda |
expressed in Escherichia coli as His-tag fusion protein | Populus tremuloides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-coumarate | strong competitive inhibitor for the other substrates | Populus tremuloides | |
caffeate | strong competitive inhibitor for the other substrates | Populus tremuloides | |
ferulate | strong competitive inhibitor for the other substrates | Populus tremuloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
4-coumarate | 4CL2, pH 7.5, 30°C | Populus tremuloides | |
0.035 | - |
caffeate | 4CL1, pH 7.5, 30°C | Populus tremuloides | |
0.042 | - |
caffeate | 4CL2, pH 7.5, 30°C | Populus tremuloides | |
0.056 | - |
4-coumarate | 4CL1, pH 7.5, 30°C | Populus tremuloides | |
0.112 | - |
ferulate | 4CL1, pH 7.5, 30°C | Populus tremuloides | |
0.139 | - |
ferulate | 4CL2, pH 7.5, 30°C | Populus tremuloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Pinus taeda | plant phenylpropanoid metabolism | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | Populus tremuloides | plant phenylpropanoid metabolism | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pinus taeda | - |
no isoforms detected, one protein with broad substrate specificity | - |
Populus tremuloides | - |
two isoforms, 4CL1 and 4CL2 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Pinus taeda |
recombinant protein using His-tag | Populus tremuloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Pinus taeda | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | - |
Populus tremuloides | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | plant phenylpropanoid metabolism | Pinus taeda | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | plant phenylpropanoid metabolism | Populus tremuloides | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Populus tremuloides | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + caffeate + CoA | same activity as with 4-coumarate | Pinus taeda | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + ferulate + CoA | only 4CL1, no substrate for 4CL2 | Populus tremuloides | AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + ferulate + CoA | same activity as with 4-coumarate | Pinus taeda | AMP + diphosphate + feruloyl-CoA | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
caffeate | 4CL1 with 4-coumarate as substrate, pH 7.5, 30°C | Populus tremuloides | |
0.012 | - |
caffeate | 4CL2 with 4-coumarate as substrate, pH 7.5, 30°C | Populus tremuloides | |
0.1 | - |
ferulate | 4CL1 with 4-coumarate as substrate, pH 7.5, 30°C | Populus tremuloides | |
0.3 | - |
ferulate | 4CL2 with 4-coumarate as substrate, pH 7.5, 30°C | Populus tremuloides |