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Literature summary for 6.2.1.12 extracted from

  • Rastogi, S.; Kumar, R.; Chanotiya, C.S.; Shanker, K.; Gupta, M.M.; Nagegowda, D.A.; Shasany, A.K.
    4-coumarate: CoA ligase partitions metabolites for eugenol biosynthesis (2013), Plant Cell Physiol., 54, 1238-1252.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons Ocimum basilicum
DNA and amino acid sequence determination and analysis, sequence comparisons Agastache rugosa
DNA and amino acid sequence determination and analysis, sequence comparisons, transient expression of OS4CL-GFP-fusion protein in Arabidopsis thaliana protoplast cytosol, expression of N-terminally His6-tagged OS4CL in Escherichia coli Ocimum tenuiflorum

Protein Variants

Protein Variants Comment Organism
additional information transient silencing of the OS4CL gene in planta by RNA interference Ocimum tenuiflorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00166
-
4-coumarate pH 7.5, 30°C Ocimum tenuiflorum
0.00302
-
ferulate pH 7.5, 30°C Ocimum tenuiflorum
0.00494
-
caffeate pH 7.5, 30°C Ocimum tenuiflorum
0.02576
-
trans-cinnamate pH 7.5, 30°C Ocimum tenuiflorum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Ocimum tenuiflorum
Mg2+ required Ocimum basilicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62480
-
x * 62480, recombinant N-terminally His6-tagged enzyme, SDS-PAGE Ocimum tenuiflorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Ocimum tenuiflorum
-
AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA Ocimum basilicum
-
AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA Ocimum basilicum CIM SAUMYA
-
AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA Ocimum tenuiflorum CIM AYU
-
AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + caffeate + CoA Ocimum tenuiflorum
-
AMP + diphosphate + caffeoyl-CoA
-
?
ATP + caffeate + CoA Ocimum tenuiflorum CIM AYU
-
AMP + diphosphate + caffeoyl-CoA
-
?
ATP + ferulate + CoA Ocimum tenuiflorum
-
AMP + diphosphate + feruloyl-CoA
-
?
ATP + ferulate + CoA Ocimum tenuiflorum CIM AYU
-
AMP + diphosphate + feruloyl-CoA
-
?
ATP + trans-cinnamate + CoA Ocimum tenuiflorum
-
AMP + diphosphate + trans-cinnamoyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Agastache rugosa Q6PQ01
-
-
Ocimum basilicum S4WX39 isozyme OB4CL_ctg4
-
Ocimum basilicum CIM SAUMYA S4WX39 isozyme OB4CL_ctg4
-
Ocimum tenuiflorum T1NXG6 i.e. Ocimum tenuiflorum, isozyme OS4CL
-
Ocimum tenuiflorum CIM AYU T1NXG6 i.e. Ocimum tenuiflorum, isozyme OS4CL
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Ocimum tenuiflorum
-
leaf
-
Ocimum basilicum
-
additional information quantitative reverse transcription-PCR expression analysis at different developmental stages, 2, 4 and 6 weeks after seed germination, overview Ocimum tenuiflorum
-
root
-
Ocimum tenuiflorum
-
root
-
Ocimum basilicum
-
stem
-
Ocimum tenuiflorum
-
stem
-
Ocimum basilicum
-
trichome glandular trichome-rich tissue Ocimum tenuiflorum
-
trichome glandular trichome-rich tissue Ocimum basilicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-coumarate + CoA
-
Ocimum tenuiflorum AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA
-
Ocimum basilicum AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA best substrate Ocimum tenuiflorum AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA
-
Ocimum basilicum CIM SAUMYA AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA
-
Ocimum tenuiflorum CIM AYU AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + 4-coumarate + CoA best substrate Ocimum tenuiflorum CIM AYU AMP + diphosphate + 4-coumaroyl-CoA
-
?
ATP + caffeate + CoA
-
Ocimum tenuiflorum AMP + diphosphate + caffeoyl-CoA
-
?
ATP + caffeate + CoA
-
Ocimum tenuiflorum CIM AYU AMP + diphosphate + caffeoyl-CoA
-
?
ATP + ferulate + CoA
-
Ocimum tenuiflorum AMP + diphosphate + feruloyl-CoA
-
?
ATP + ferulate + CoA
-
Ocimum tenuiflorum CIM AYU AMP + diphosphate + feruloyl-CoA
-
?
ATP + trans-cinnamate + CoA
-
Ocimum tenuiflorum AMP + diphosphate + trans-cinnamoyl-CoA
-
?
additional information no activity with sinapic acid Ocimum tenuiflorum ?
-
?
additional information no activity with sinapic acid Ocimum tenuiflorum CIM AYU ?
-
?

Subunits

Subunits Comment Organism
? x * 62480, recombinant N-terminally His6-tagged enzyme, SDS-PAGE Ocimum tenuiflorum

Synonyms

Synonyms Comment Organism
4-coumarate: CoA ligase
-
Ocimum tenuiflorum
4-coumarate: CoA ligase
-
Ocimum basilicum
4-coumarate: CoA ligase
-
Agastache rugosa
4-coumarate:CoA ligase
-
Ocimum tenuiflorum
4-coumarate:CoA ligase
-
Ocimum basilicum
4CL
-
Ocimum tenuiflorum
4CL
-
Ocimum basilicum
Ar4CL
-
Agastache rugosa
OB4CL_ctg4
-
Ocimum basilicum
OS4CL
-
Ocimum tenuiflorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Ocimum basilicum
22 30 assay at Ocimum tenuiflorum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
caffeate pH 7.5, 30°C Ocimum tenuiflorum
0.58
-
trans-cinnamate pH 7.5, 30°C Ocimum tenuiflorum
0.7 1 4-coumarate pH 7.5, 30°C Ocimum tenuiflorum
1.56
-
ferulate pH 7.5, 30°C Ocimum tenuiflorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Ocimum tenuiflorum
7.5
-
assay at Ocimum basilicum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Ocimum tenuiflorum
ATP
-
Ocimum basilicum

General Information

General Information Comment Organism
evolution the enzyme belongs to the class II 4-coumarate-CoA ligases Ocimum tenuiflorum
evolution the enzyme belongs to the class II 4-coumarate-CoA ligases Ocimum basilicum
evolution the enzyme belongs to the class II 4-coumarate-CoA ligases Agastache rugosa
malfunction a significant reduction in the expression levels is observed for OB4CL_ctg4-related transcripts in suppressed trichome compared with transcripts similar to the other four isoforms, OB4CL_ctg1, 2, 3 and 5 Ocimum basilicum
malfunction transient RNAi suppression of OS4CL in leaves causes a reduction in leaf eugenol content and trichome transcript level, with a considerable increase in endogenous 4-coumaric, ferulic, trans-cinnamic and caffeic acids, but no significant effect of OS4CL suppression on the total lignin content Ocimum tenuiflorum
metabolism the enzyme is involved in the biosynthetic pathway of eugenol/chavicol synthesis in Ocimum sanctum, overview Ocimum tenuiflorum
physiological function metabolite channeling of intermediates towards eugenol by a specific 4-coumarate:CoA ligase and involvement of the enzyme in creation of virtual compartments through substrate utilization and committing metabolites for eugenol biosynthesis at an early stage of the pathway, overview Ocimum tenuiflorum