Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons | Ocimum basilicum |
DNA and amino acid sequence determination and analysis, sequence comparisons | Agastache rugosa |
DNA and amino acid sequence determination and analysis, sequence comparisons, transient expression of OS4CL-GFP-fusion protein in Arabidopsis thaliana protoplast cytosol, expression of N-terminally His6-tagged OS4CL in Escherichia coli | Ocimum tenuiflorum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | transient silencing of the OS4CL gene in planta by RNA interference | Ocimum tenuiflorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00166 | - |
4-coumarate | pH 7.5, 30°C | Ocimum tenuiflorum | |
0.00302 | - |
ferulate | pH 7.5, 30°C | Ocimum tenuiflorum | |
0.00494 | - |
caffeate | pH 7.5, 30°C | Ocimum tenuiflorum | |
0.02576 | - |
trans-cinnamate | pH 7.5, 30°C | Ocimum tenuiflorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Ocimum tenuiflorum | |
Mg2+ | required | Ocimum basilicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62480 | - |
x * 62480, recombinant N-terminally His6-tagged enzyme, SDS-PAGE | Ocimum tenuiflorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Ocimum tenuiflorum | - |
AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | Ocimum basilicum | - |
AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | Ocimum basilicum CIM SAUMYA | - |
AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | Ocimum tenuiflorum CIM AYU | - |
AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | Ocimum tenuiflorum | - |
AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + caffeate + CoA | Ocimum tenuiflorum CIM AYU | - |
AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + ferulate + CoA | Ocimum tenuiflorum | - |
AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + ferulate + CoA | Ocimum tenuiflorum CIM AYU | - |
AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + trans-cinnamate + CoA | Ocimum tenuiflorum | - |
AMP + diphosphate + trans-cinnamoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agastache rugosa | Q6PQ01 | - |
- |
Ocimum basilicum | S4WX39 | isozyme OB4CL_ctg4 | - |
Ocimum basilicum CIM SAUMYA | S4WX39 | isozyme OB4CL_ctg4 | - |
Ocimum tenuiflorum | T1NXG6 | i.e. Ocimum tenuiflorum, isozyme OS4CL | - |
Ocimum tenuiflorum CIM AYU | T1NXG6 | i.e. Ocimum tenuiflorum, isozyme OS4CL | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Ocimum tenuiflorum | - |
leaf | - |
Ocimum basilicum | - |
additional information | quantitative reverse transcription-PCR expression analysis at different developmental stages, 2, 4 and 6 weeks after seed germination, overview | Ocimum tenuiflorum | - |
root | - |
Ocimum tenuiflorum | - |
root | - |
Ocimum basilicum | - |
stem | - |
Ocimum tenuiflorum | - |
stem | - |
Ocimum basilicum | - |
trichome | glandular trichome-rich tissue | Ocimum tenuiflorum | - |
trichome | glandular trichome-rich tissue | Ocimum basilicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Ocimum tenuiflorum | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | - |
Ocimum basilicum | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | best substrate | Ocimum tenuiflorum | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | - |
Ocimum basilicum CIM SAUMYA | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | - |
Ocimum tenuiflorum CIM AYU | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | best substrate | Ocimum tenuiflorum CIM AYU | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Ocimum tenuiflorum | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Ocimum tenuiflorum CIM AYU | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + ferulate + CoA | - |
Ocimum tenuiflorum | AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + ferulate + CoA | - |
Ocimum tenuiflorum CIM AYU | AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + trans-cinnamate + CoA | - |
Ocimum tenuiflorum | AMP + diphosphate + trans-cinnamoyl-CoA | - |
? | |
additional information | no activity with sinapic acid | Ocimum tenuiflorum | ? | - |
? | |
additional information | no activity with sinapic acid | Ocimum tenuiflorum CIM AYU | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62480, recombinant N-terminally His6-tagged enzyme, SDS-PAGE | Ocimum tenuiflorum |
Synonyms | Comment | Organism |
---|---|---|
4-coumarate: CoA ligase | - |
Ocimum tenuiflorum |
4-coumarate: CoA ligase | - |
Ocimum basilicum |
4-coumarate: CoA ligase | - |
Agastache rugosa |
4-coumarate:CoA ligase | - |
Ocimum tenuiflorum |
4-coumarate:CoA ligase | - |
Ocimum basilicum |
4CL | - |
Ocimum tenuiflorum |
4CL | - |
Ocimum basilicum |
Ar4CL | - |
Agastache rugosa |
OB4CL_ctg4 | - |
Ocimum basilicum |
OS4CL | - |
Ocimum tenuiflorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Ocimum basilicum |
22 | 30 | assay at | Ocimum tenuiflorum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
caffeate | pH 7.5, 30°C | Ocimum tenuiflorum | |
0.58 | - |
trans-cinnamate | pH 7.5, 30°C | Ocimum tenuiflorum | |
0.7 | 1 | 4-coumarate | pH 7.5, 30°C | Ocimum tenuiflorum | |
1.56 | - |
ferulate | pH 7.5, 30°C | Ocimum tenuiflorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Ocimum tenuiflorum |
7.5 | - |
assay at | Ocimum basilicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Ocimum tenuiflorum | |
ATP | - |
Ocimum basilicum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the class II 4-coumarate-CoA ligases | Ocimum tenuiflorum |
evolution | the enzyme belongs to the class II 4-coumarate-CoA ligases | Ocimum basilicum |
evolution | the enzyme belongs to the class II 4-coumarate-CoA ligases | Agastache rugosa |
malfunction | a significant reduction in the expression levels is observed for OB4CL_ctg4-related transcripts in suppressed trichome compared with transcripts similar to the other four isoforms, OB4CL_ctg1, 2, 3 and 5 | Ocimum basilicum |
malfunction | transient RNAi suppression of OS4CL in leaves causes a reduction in leaf eugenol content and trichome transcript level, with a considerable increase in endogenous 4-coumaric, ferulic, trans-cinnamic and caffeic acids, but no significant effect of OS4CL suppression on the total lignin content | Ocimum tenuiflorum |
metabolism | the enzyme is involved in the biosynthetic pathway of eugenol/chavicol synthesis in Ocimum sanctum, overview | Ocimum tenuiflorum |
physiological function | metabolite channeling of intermediates towards eugenol by a specific 4-coumarate:CoA ligase and involvement of the enzyme in creation of virtual compartments through substrate utilization and committing metabolites for eugenol biosynthesis at an early stage of the pathway, overview | Ocimum tenuiflorum |