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Literature summary for 6.2.1.12 extracted from

  • Liu, T.; Yao, R.; Zhao, Y.; Xu, S.; Huang, C.; Luo, J.; Kong, L.
    Cloning, functional characterization and site-directed mutagenesis of 4-coumarate coenzyme A ligase (4CL) involved in coumarin biosynthesis in Peucedanum praeruptorum Dunn (2017), Front. Plant Sci., 8, 4-4 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Peucedanum praeruptorum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Peucedanum praeruptorum 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Peucedanum praeruptorum
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Peucedanum praeruptorum A0A1L7H9I4
-
-
Peucedanum praeruptorum A0A1L7H9K0
-
-
Peucedanum praeruptorum A0A1L7H9K1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Peucedanum praeruptorum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Peucedanum praeruptorum
-
root
-
 Peucedanum praeruptorum
-
root isoform 4CL1 is the most highly expressed gene in roots Peucedanum praeruptorum
-
seedling
-
 Peucedanum praeruptorum
-
stem
-
 Peucedanum praeruptorum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-coumarate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + 2-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + caffeate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + cinnamate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + cinnamoyl-CoA
-
 ?
ATP + ferulate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + feruloyl-CoA
-
 ?
ATP + isoferulate + CoA
-
 Peucedanum praeruptorum AMP + diphosphate + isoferuloyl-CoA
-
 ?
additional information isoform 4CL10 has no catalytic activity toward hydroxycinnamic acid compounds Peucedanum praeruptorum ?
-
 ?
additional information isoform 4CL7 has no catalytic activity toward hydroxycinnamic acid compounds Peucedanum praeruptorum ?
-
 ?

Synonyms

Synonyms Comment Organism
4-coumarate: coenzyme A ligase
-
 Peucedanum praeruptorum
4CL1 isoform Peucedanum praeruptorum
4CL10 isoform Peucedanum praeruptorum
4CL7 isoform Peucedanum praeruptorum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Peucedanum praeruptorum

Expression

Organism Comment Expression
Peucedanum praeruptorum upon PEG, NaCl, CuSO4, heat and cold treatments, the expression level of isoform 4CL1 significantly decreases down
Peucedanum praeruptorum the expression level of isoform 4CL10 is clearly enhanced under PEG treatment up
Peucedanum praeruptorum transcript abundance of isoform 4CL7 is up-regulated under heat treatment up