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Literature summary for 6.2.1.17 extracted from

  • Rajashekhara, E.; Watanabe, K.
    Propionyl-coenzyme A synthetases of Ralstonia solanacearum and Salmonella choleraesuis display atypical kinetics (2003), FEBS Lett., 556, 143-147.
    View publication on PubMed
Search for more literature for 6.2.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) Salmonella enterica
expressed in Escherichia coli Rosetta(DE3) Ralstonia solanacearum
expression in Escherichia coli Salmonella enterica
expression in Escherichia coli Ralstonia solanacearum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 propionate pH 8.0, 37°C Salmonella enterica
0.08
-
 propionate 45°C, pH 7.0 Salmonella enterica
0.26
-
 propionate 37°C, pH 8.0 Ralstonia solanacearum
0.26
-
 propionate pH 8.0, 37°C Ralstonia solanacearum
0.33
-
 ATP pH 8.0, 37°C Salmonella enterica
0.33
-
 ATP 45°C, pH 7.0, cosubstrate: propanoate Salmonella enterica
0.65
-
 ATP pH 8.0, 37°C Ralstonia solanacearum
0.65
-
 ATP 37°C, pH 8.0, cosubstrate: propanoate Ralstonia solanacearum
0.69
-
 CoA pH 8.0, 37°C Ralstonia solanacearum
0.69
-
 CoA 37°C, pH 8.0, cosubstrate: propanoate Ralstonia solanacearum
0.9
-
 CoA pH 8.0, 37°C Salmonella enterica
0.9
-
 CoA 45°C, pH 7.0, cosubstrate: propanoate Salmonella enterica
1
-
 Acrylate 37°C, pH 8.0 Ralstonia solanacearum
1
-
 Acrylate pH 8.0, 37°C Ralstonia solanacearum
1.3
-
 Acrylate pH 8.0, 37°C Salmonella enterica
1.3
-
 Acrylate 45°C, pH 7.0 Salmonella enterica
1.9
-
 acetate 37°C, pH 8.0 Ralstonia solanacearum
1.9
-
 acetate pH 8.0, 37°C Salmonella enterica
1.9
-
 acetate pH 8.0, 37°C Ralstonia solanacearum
1.9
-
 acetate 45°C, pH 7.0 Salmonella enterica
13
-
 Butyrate 37°C, pH 8.0 Ralstonia solanacearum
13
-
 Butyrate pH 8.0, 37°C Ralstonia solanacearum
82
-
 Butyrate pH 8.0, 37°C Salmonella enterica
82
-
 Butyrate 45°C, pH 7.0 Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 SDS-PAGE, gel filtration Salmonella enterica
70000
-
 SDS-PAGE, gel filtration Ralstonia solanacearum
70900
-
 calculated from amino acid sequence Ralstonia solanacearum
71300
-
 calculated from amino acid sequence Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + propanoate + CoA Salmonella enterica required for propionate catabolism AMP + diphosphate + propanoyl-CoA
-
 ?
ATP + propanoate + CoA Ralstonia solanacearum required for propionate catabolism AMP + diphosphate + propanoyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Ralstonia solanacearum
-
-
-
Salmonella enterica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Salmonella enterica
recombinant Ralstonia solanacearum
recombinant enzyme using His-tag Salmonella enterica
recombinant enzyme using His-tag Ralstonia solanacearum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA
-
 Salmonella enterica AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA
-
 Ralstonia solanacearum AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA 128% of the activity with propanoate Salmonella enterica AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA 150% of the activity with propanoate Ralstonia solanacearum AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acrylate + CoA
-
 Salmonella enterica AMP + diphosphate + acryloyl-CoA
-
 ?
ATP + acrylate + CoA
-
 Ralstonia solanacearum AMP + diphosphate + acryloyl-CoA
-
 ?
ATP + acrylate + CoA 109% of the activity with propanoate Ralstonia solanacearum AMP + diphosphate + acryloyl-CoA
-
 ?
ATP + acrylate + CoA 20% of the activity with propanoate Salmonella enterica AMP + diphosphate + acryloyl-CoA
-
 ?
ATP + butyrate + CoA
-
 Salmonella enterica AMP + diphosphate + butyryl-CoA
-
 ?
ATP + butyrate + CoA
-
 Ralstonia solanacearum AMP + diphosphate + butyryl-CoA
-
 ?
ATP + butyrate + CoA 14% of the activity with propanoate Salmonella enterica AMP + diphosphate + butyryl-CoA
-
 ?
ATP + butyrate + CoA 60% of the activity with propanoate Ralstonia solanacearum AMP + diphosphate + butyryl-CoA
-
 ?
ATP + propanoate + CoA
-
 Salmonella enterica AMP + diphosphate + propanoyl-CoA
-
 ?
ATP + propanoate + CoA
-
 Ralstonia solanacearum AMP + diphosphate + propanoyl-CoA
-
 ?
ATP + propanoate + CoA required for propionate catabolism Salmonella enterica AMP + diphosphate + propanoyl-CoA
-
 ?
ATP + propanoate + CoA required for propionate catabolism Ralstonia solanacearum AMP + diphosphate + propanoyl-CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, equal molecular weights in SDS-PAGE and gel filtration Salmonella enterica
monomer 1 * 70000, equal molecular weights in SDS-PAGE and gel filtration Ralstonia solanacearum

Synonyms

Synonyms Comment Organism
Propionyl-coenzyme A synthetase
-
 Salmonella enterica
Propionyl-coenzyme A synthetase
-
 Ralstonia solanacearum
PrpE
-
 Salmonella enterica
PrpE
-
 Ralstonia solanacearum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Ralstonia solanacearum
45
-
-
 Salmonella enterica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 unstable above 40°C Salmonella enterica
40
-
 unstable above 40°C Ralstonia solanacearum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.87
-
 Butyrate pH 8.0, 37°C Salmonella enterica
0.87
-
 Butyrate 45°C, pH 7.0 Salmonella enterica
1
-
 CoA pH 8.0, 37°C Salmonella enterica
1
-
 CoA 45°C, pH 7.0, cosubstrate: propanoate Salmonella enterica
1.1
-
 ATP pH 8.0, 37°C Salmonella enterica
1.1
-
 Acrylate pH 8.0, 37°C Salmonella enterica
1.1
-
 Acrylate 45°C, pH 7.0 Salmonella enterica
1.1
-
 ATP 45°C, pH 7.0: cosubstrate: propanoate Salmonella enterica
1.2
-
 propionate pH 8.0, 37°C Salmonella enterica
1.2
-
 propionate 45°C, pH 7.0 Salmonella enterica
1.3
-
 Acrylate pH 8.0, 37°C Ralstonia solanacearum
1.3
-
 Acrylate 37°C, pH 8.0 Ralstonia solanacearum
1.5
-
 ATP pH 8.0, 37°C Ralstonia solanacearum
1.5
-
 ATP 37°C, pH 8.0, cosubstrate: propanoate Ralstonia solanacearum
1.7
-
 Butyrate pH 8.0, 37°C Ralstonia solanacearum
1.7
-
 Butyrate 37°C, pH 8.0 Ralstonia solanacearum
1.8
-
 propionate pH 8.0, 37°C Ralstonia solanacearum
1.8
-
 propionate 37°C, pH 8.0 Ralstonia solanacearum
2.4
-
 CoA pH 8.0, 37°C Ralstonia solanacearum
2.4
-
 CoA 37°C, pH 8.0, cosubstrate: propanoate Ralstonia solanacearum
4.8
-
 acetate pH 8.0, 37°C Salmonella enterica
4.8
-
 acetate 45°C, pH 7.0 Salmonella enterica
6.8
-
 acetate pH 8.0, 37°C Ralstonia solanacearum
6.8
-
 acetate 37°C, pH 8.0 Ralstonia solanacearum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Salmonella enterica
7
-
 at 45°C Salmonella enterica
8
-
-
 Ralstonia solanacearum
8
-
 at 37°C Ralstonia solanacearum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 almost complete loss of activity within 30 min Salmonella enterica
5
-
 almost complete loss of activity within 30 min Ralstonia solanacearum
9
-
 stable Salmonella enterica
9
-
 stable Ralstonia solanacearum