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Literature summary for 6.2.1.2 extracted from
- Purification, characterization, and mass spectrometric sequencing of a medium chain acyl-CoA synthetase from mouse liver mitochondria and comparisons with the homologs of rat and bovine (2006), Protein Expr. Purif., 47, 405-414.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diflunisal | 0.001 mM, complete inhibition | Bos taurus |  |
| diflunisal | 0.001 mM, complete inhibition | Mus musculus | |
| enoxacin | - |
Bos taurus | |
| enoxacin | - |
Mus musculus | |
| Nalidixic acid | 0.001 mM, complete inhibition | Bos taurus | |
| Nalidixic acid | 0.001 mM, complete inhibition | Mus musculus | |
| ofloxacine | - |
Bos taurus | |
| ofloxacine | - |
Mus musculus | |
| salicylic acid | 0.001 mM, complete inhibition | Bos taurus | |
| salicylic acid | 0.001 mM, complete inhibition | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mus musculus | 5739 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
SDS-PAGE | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephadex G-25 gel filtration, DEAE-Sepharose column chromatography, and hydroxylapatite column chromatography | Mus musculus |
| Sephadex G-25 gel filtration, DEAE-Sepharose column chromatography, and hydroxylapatite column chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Mus musculus | - |
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.023 | - |
cell extract, with 0.001 mM hexanoic acid as a substrate in 0.2 M Tris-HCl buffer (pH 8.5) | Mus musculus |
| 4.14 | - |
after 180fold purification, with 0.001 mM hexanoic acid as a substrate in 0.2 M Tris-HCl buffer (pH 8.5) | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-naphthoic acid + CoA | - |
Mus musculus | AMP + diphosphate + 1-naphthoyl-CoA | - |
? | |
| ATP + 1-naphthoic acid + CoA | - |
Bos taurus | AMP + diphosphate + 1-naphthoyl-CoA | - |
? | |
| ATP + 1-naphthylacetic acid + CoA | - |
Mus musculus | AMP + diphosphate + 1-naphthylacetoyl-CoA | - |
? | |
| ATP + 1-naphthylacetic acid + CoA | - |
Bos taurus | AMP + diphosphate + 1-naphthylacetoyl-CoA | - |
? | |
| ATP + 3-methylbenzoate + CoA | - |
Mus musculus | AMP + diphosphate + 3-methylbenzoyl-CoA | - |
? | |
| ATP + 3-methylbenzoate + CoA | - |
Bos taurus | AMP + diphosphate + 3-methylbenzoyl-CoA | - |
? | |
| ATP + 4-methylbenzoate + CoA | - |
Mus musculus | AMP + diphosphate + 4-methylbenzoyl-CoA | - |
? | |
| ATP + 4-methylbenzoate + CoA | - |
Bos taurus | AMP + diphosphate + 4-methylbenzoyl-CoA | - |
? | |
| ATP + benzoate + CoA | high activities are obtained with benzoate having methyl, pentyl, and methoxy groups in the para- or meta-positions of the benzene ring | Mus musculus | AMP + diphosphate + benzoyl-CoA | - |
? | |
| ATP + benzoate + CoA | high activities are obtained with benzoate having methyl, pentyl, and methoxy groups in the para- or meta-positions of the benzene ring | Bos taurus | AMP + diphosphate + benzoyl-CoA | - |
? | |
| ATP + decanoate + CoA | - |
Mus musculus | AMP + diphosphate + decanoyl-CoA | - |
? | |
| ATP + decanoate + CoA | - |
Bos taurus | AMP + diphosphate + decanoyl-CoA | - |
? | |
| ATP + hexanoate + CoA | maximal activity on hexanoate | Mus musculus | AMP + diphosphate + hexanoyl-CoA | - |
? | |
| ATP + hexanoate + CoA | maximal activity on hexanoate | Bos taurus | AMP + diphosphate + hexanoyl-CoA | - |
? | |
| ATP + ketoprofen + CoA | less active on ketoprofen | Mus musculus | ADP + diphosphate + ? | - |
? | |
| ATP + ketoprofen + CoA | less active on ketoprofen | Bos taurus | ADP + diphosphate + ? | - |
? | |
| ATP + m-methoxybenzoate + CoA | - |
Mus musculus | AMP + diphosphate + m-methoxybenzoyl-CoA | - |
? | |
| ATP + m-methoxybenzoate + CoA | - |
Bos taurus | AMP + diphosphate + m-methoxybenzoyl-CoA | - |
? | |
| ATP + m-pentylbenzoate + CoA | - |
Mus musculus | AMP + diphosphate + m-pentylbenzoyl-CoA | - |
? | |
| ATP + m-pentylbenzoate + CoA | - |
Bos taurus | AMP + diphosphate + m-pentylbenzoyl-CoA | - |
? | |
| ATP + octanoate + CoA | - |
Mus musculus | AMP + diphosphate + octanoyl-CoA | - |
? | |
| ATP + octanoate + CoA | - |
Bos taurus | AMP + diphosphate + octanoyl-CoA | - |
? | |
| ATP + p-methoxybenzoate + CoA | - |
Mus musculus | AMP + diphosphate + p-methoxybenzoyl-CoA | - |
? | |
| ATP + p-methoxybenzoate + CoA | - |
Bos taurus | AMP + diphosphate + p-methoxybenzoyl-CoA | - |
? | |
| ATP + p-pentylbenzoate + CoA | - |
Mus musculus | AMP + diphosphate + p-pentylbenzoyl-CoA | - |
? | |
| ATP + p-pentylbenzoate + CoA | - |
Bos taurus | AMP + diphosphate + p-pentylbenzoyl-CoA | - |
? | |
| ATP + valproate + CoA | less active on valproate | Mus musculus | ADP + diphosphate + valproyl-CoA | - |
? | |
| ATP + valproate + CoA | less active on valproate | Bos taurus | ADP + diphosphate + valproyl-CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| medium chain acyl-CoA synthetase | - |
Mus musculus |
| medium chain acyl-CoA synthetase | - |
Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0237 | - |
enoxacin | - |
Mus musculus | |
| 0.0382 | - |
ofloxacine | - |
Mus musculus | |
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