Any feedback?
Literature summary for 6.2.1.20 extracted from
- Acyl-acyl carrier protein synthetase from Escherichia coli (1981), Methods Enzymol., 71, 163-168.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is a useful tool for the synthesis of native acyl-[acyl-carrier-protein] for use in examining other lipid biosynthetic enzymes in E. coli | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | 0.015 | saturated fatty acids | C12:0 to C16:0 | Escherichia coli | |
| 0.03 | 0.16 | unsaturated fatty acids | fatty acids with shorter chain length than 12 | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| inner membrane | - |
Escherichia coli | - |
- |
| membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli |  |
| Salt | requirement for high concentrations of salt, with the exception of Mg2+, the requirement for ion is nonspecific. Chaotrophic salts are effective at lower concentrations than less chaotrophic salts | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | enzyme may function to reintroduce free fatty acids into the biosynthetic pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + an acid + [acyl-carrier protein] | the best substrates are saturated fatty acids, C12:0 to C16:0 | Escherichia coli | AMP + diphosphate + acyl-[acyl-carrier protein] | - |
? | |
| ATP + palmitic acid + [acyl-carrier protein] | - |
Escherichia coli | AMP + diphosphate + palmitoyl-[acyl-carrier protein] | - |
? | |
| ATP + saturated fatty acids + an [acyl-carrier protein] | - |
Escherichia coli | ? | - |
? | |
| ATP + unsaturated fatty acids + an [acyl-carrier protein] | - |
Escherichia coli | ? | - |
? | |
| additional information | enzyme may function to reintroduce free fatty acids into the biosynthetic pathway | Escherichia coli | ? | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
