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Literature summary for 6.2.1.25 extracted from

  • Schuhle, K.; Gescher, J.; Feil, U.; Paul, M.; Jahn, M.; Schagger, H.; Fuchs, G.
    Benzoate-coenzyme A ligase from Thauera aromatica: an enzyme acting in anaerobic and aerobic pathways (2003), J. Bacteriol., 185, 4920-4929.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thauera aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 benzoate 37°C Thauera aromatica
0.15
-
 2-Aminobenzoate 37°C Thauera aromatica
0.18
-
 CoASH 37°C Thauera aromatica
0.44
-
 ATP 37°C Thauera aromatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 gel filtration Thauera aromatica

Organism

Organism UniProt Comment Textmining
Thauera aromatica
-
 strain DSM 6984
-

Purification (Commentary)

Purification (Comment) Organism
at 4°C, by a method that includes DEAE-sepharose, source 30Q and reactive green chromatography Thauera aromatica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.5
-
 37°C Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-aminobenzoate + CoA 60% of the activity relative to benzoate Thauera aromatica AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
ATP + 2-fluorobenzoate + CoA 122% of the activity relative to benzoate Thauera aromatica AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
ATP + 3-fluorobenzoate + CoA 89% of the activity relative to benzoate Thauera aromatica AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
ATP + 3-hydroxybenzoate + CoA
-
 Thauera aromatica AMP + diphosphate + 3-hydroxybenzoyl-CoA
-
 ?
ATP + 4-fluorobenzoate + CoA 100% of the activity relative to benzoate Thauera aromatica AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
ATP + 4-hydroxybenzoate + CoA
-
 Thauera aromatica AMP + diphosphate + 4-hydroxybenzoyl-CoA
-
 ?
ATP + acetate + CoA
-
 Thauera aromatica AMP + diphosphate + acetyl-CoA
-
 ?
ATP + benzoate + CoA
-
 Thauera aromatica AMP + diphosphate + benzoyl-CoA
-
 ?
ATP + cyclohex-1-ene-1-carboxylate + CoA 8% of the activity relative to benzoate Thauera aromatica AMP + diphosphate + cyclohex-1-ene-1-carbonyl-CoA
-
 ?
ATP + cyclohexa-1,5-diene-1-carboxylate + CoA 23% of the activity relative to benzoate Thauera aromatica AMP + diphosphate + cyclohexa-1,5-diene-1-carboxyl-CoA
-
 ?
ATP + glutarate + CoA
-
 Thauera aromatica AMP + diphosphate + glutaryl-CoA
-
 ?
ATP + phenylacetate + CoA
-
 Thauera aromatica AMP + diphosphate + phenylacetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 58000 gel filtration Thauera aromatica