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Literature summary for 6.2.1.25 extracted from

  • Thornburg, C.; Walter, T.; Walker, K.
    Biocatalysis of a paclitaxel analogue conversion of baccatin III to N-debenzoyl-N-(2-furoyl)paclitaxel and characterization of an amino phenylpropanoyl CoA transferase (2017), Biochemistry, 56, 5920-5930 .
    View publication on PubMed
Search for more literature for 6.2.1.25

Protein Variants

Protein Variants Comment Organism
additional information an enzyme cascade reaction using a benzoate CoA ligase (BadA), a modified nonribosomal peptide synthase (PheAT), a phenylpropanoyltransferase (BAPT), and a benzoyltransferase (NDTNBT) to produce an anticancer paclitaxel analogue and its precursor from the commercially available biosynthetic intermediate baccatin III is established. This reaction network has great potential for optimization to scale-up production and is attractive in its regioselective O- and N-acylation steps that remove protecting group manipulations used in paclitaxel analogue synthesis Taxus baccata

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Taxus baccata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + benzoate + CoA Taxus baccata
-
 AMP + diphosphate + benzoyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus baccata
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + benzoate + CoA
-
 Taxus baccata AMP + diphosphate + benzoyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
BadA
-
 Taxus baccata
benzoate-CoA ligase
-
 Taxus baccata

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Taxus baccata