Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.26 extracted from

  • Kwon, O.; Bhattacharyya, D.K.; Meganathan, R.
    Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and properties of o-succinylbenzoyl-coenzyme A synthetase from Escherichia coli (1996), J. Bacteriol., 178, 6778-6781.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.26

Inhibitors

Inhibitors Comment Organism Structure
Fe2+
-
 Escherichia coli
Hg2+
-
 Escherichia coli
Mg2+ above 1 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 o-succinylbenzoate
-
 Escherichia coli
0.0735
-
 ATP
-
 Escherichia coli
0.36
-
 CoA
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.5 mM, 1 mM or 5 mM, approximately twofold higher activity than the control Escherichia coli
Co2+ at 0.5 mM or 1.0 mM, 4fold increase in activity, 2fold increase in activity at 5 mM Escherichia coli
K+ 0.5 mM, 1 mM or 5 mM, approximately twofold higher activity than the control Escherichia coli
Mg2+ maximal activation at 1 mM Mg2+, 6fold Escherichia coli
Mn2+ at 0.5 mM or 1.0 mM, 4fold increase in activity, 2fold increase in activity at 5 mM Escherichia coli
Na+ 0.5 mM, 1 mM or 5 mM, approximately twofold higher activity than the control Escherichia coli
Zn2+ 0.5 mM, 1 mM or 5 mM, approximately twofold higher activity than the control Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 4 * 49000, SDS-PAGE Escherichia coli
185000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-succinylbenzoate + CoA Escherichia coli o-succinylbenzoyl-CoA is an intermediate in menaquinone (vitamin K2) biosynthesis AMP + diphosphate + 2-succinylbenzoyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-succinylbenzoate + CoA
-
 Escherichia coli AMP + diphosphate + 2-succinylbenzoyl-CoA o-succinylbenzoyl-CoA is a highly unstable compound which spontaneously converts to o-succinylbenzoate-spirolactone in absence of 1,4-dihydroxy-2-naphthoic acid synthase ?
ATP + 2-succinylbenzoate + CoA o-succinylbenzoyl-CoA is an intermediate in menaquinone (vitamin K2) biosynthesis Escherichia coli AMP + diphosphate + 2-succinylbenzoyl-CoA
-
 ?
additional information no activity with: o-malonylbenzoic acid, benzoylpropionic acid, benzoic acid, o-acetylbenzoic acid, phthalic acid and p-coumaric acid Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 49000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: 55% of maximal activity, pH 8.5: 80% of maximnal activity Escherichia coli