Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | E2, no effect on isoenzyme E3 | Pseudomonas sp. | |
Cu2+ | - |
Pseudomonas sp. | |
n-octylglucoside | - |
Pseudomonas sp. | |
PCMB | isoenzyme E3, no effect on E2 | Pseudomonas sp. | |
SDS | - |
Pseudomonas sp. | |
Tween 100 | - |
Pseudomonas sp. | |
Zn2+ | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
2-Aminobenzoate | isoenzyme E2 | Pseudomonas sp. | |
0.015 | - |
2-Aminobenzoate | isoenzyme E3 | Pseudomonas sp. | |
0.02 | - |
CoA | isoenzyme E2 | Pseudomonas sp. | |
0.026 | - |
CoA | isoenzyme E3 | Pseudomonas sp. | |
0.035 | - |
benzoate | isoenzyme E2 | Pseudomonas sp. | |
0.04 | - |
ATP | isoenzyme E3 | Pseudomonas sp. | |
0.05 | - |
2-Fluorobenzoate | isoenzyme E2 | Pseudomonas sp. | |
0.07 | - |
2-Fluorobenzoate | isoenzyme E3 | Pseudomonas sp. | |
0.075 | - |
benzoate | isoenzyme E3 | Pseudomonas sp. | |
0.083 | - |
ATP | isoenzyme E2 | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas sp. | |
Mn2+ | required, can replace Mg2+ | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
isoenzyme E2, gel filtration | Pseudomonas sp. |
65000 | - |
isoenzyme E3, gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + anthranilate + CoA | Pseudomonas sp. | isoenzyme E2 is induced anaerobically by 2-aminobenzoate and plays a role in anaerobic metabolism, E3 is induced aerobically by 2-aminobenzoate and is a key enzyme of aerobic metabolism | AMP + diphosphate + anthranilyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. KB740 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
purified enzymes not sensitive to oxygen | Pseudomonas sp. |
Purification (Comment) | Organism |
---|---|
strain KB740, 2 forms, E2: MW 60000, induced anaerobically by 2-aminobenzoate and E3: MW 65000, induced aerobically by 2-aminobenzoate | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:2-aminobenzoate-grown cell | anaerob and nitrate | Pseudomonas sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
4°C, 0.05 mg/ml protein concentration, Tris-HCl buffer, pH 7.8, 50% loss of activity after 4 days | Pseudomonas sp. |
frozen in liquid nitrogen for 3 months without remarkable loss of activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-fluorobenzoate + CoA | at the same reaction rate as 2-aminobenzoate | Pseudomonas sp. | AMP + 2-fluorobenzoyl-CoA + diphosphate | - |
? | |
ATP + 2-methylbenzoate + CoA | at less than 1/5 of the rate as 2-aminobenzoate | Pseudomonas sp. | AMP + 2-methylbenzoyl-CoA + diphosphate | - |
? | |
ATP + 3-fluorobenzoate + CoA | at half the reaction rate as 2-aminobenzoate | Pseudomonas sp. | AMP + 3-fluorobenzoyl-CoA + diphosphate | - |
? | |
ATP + 4-fluorobenzoate + CoA | at the same reaction rate as 2-aminobenzoate | Pseudomonas sp. | AMP + 4-fluorobenzoyl-CoA + diphosphate | - |
? | |
ATP + anthranilate + CoA | isoenzyme E2 is induced anaerobically by 2-aminobenzoate and plays a role in anaerobic metabolism, E3 is induced aerobically by 2-aminobenzoate and is a key enzyme of aerobic metabolism | Pseudomonas sp. | AMP + diphosphate + anthranilyl-CoA | - |
? | |
ATP + benzoate + CoA | at the same reaction rate as 2-aminobenzoate | Pseudomonas sp. | AMP + benzoyl-CoA + diphosphate | - |
? | |
ATP + CoA + anthranilate | i.e. 2-aminobenzoate | Pseudomonas sp. | AMP + diphosphate + anthranilyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, isoenzyme E2, 1 * 65000, isoenzyme E3, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.7 | - |
2-Aminobenzoate | 28°C, isoenzyme E3 | Pseudomonas sp. | |
267 | - |
2-Aminobenzoate | 28°C, isoenzyme E2 | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas sp. |
8.6 | - |
isoenzyme E3 | Pseudomonas sp. |