Cloned (Comment) | Organism |
---|---|
expression of the C-terminal His6-tagged protein in Escherichia coli BL21 | Thauera aromatica |
the gene product is expressed as a C-terminal His10-tagged protein in Escherichia coli BL21(DE3) | Azoarcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
3-hydroxybenzoate | pH and temperature not specified in the publication | Thauera aromatica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53694 | - |
x * 53694, calculated from sequence | Azoarcus sp. |
54000 | - |
x * 54000, SDS-PAGE | Azoarcus sp. |
57500 | - |
x * 57500, SDS-PAGE | Thauera aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxybenzoate + CoA | Thauera aromatica | the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27) | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azoarcus sp. | Q5P0J2 | - |
- |
Azoarcus sp. EbN1 | Q5P0J2 | - |
- |
Thauera aromatica | Q9AJS8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thauera aromatica |
- |
Azoarcus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | 3-hydroxybenzoate-CoA ligase is detected not only in 3-hydroxybenzoate-grown cells but also in catechol- and protocatechuate-grown cells. The enzyme is not detected in phenol-, 4-hydroxybenzoate-, and benzoate-grown cells | Thauera aromatica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.3 | - |
pH and temperature not specified in the publication | Thauera aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27) | Thauera aromatica | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
ATP + 3-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27). kcat/Km for 4-hydroxybenzoate is 75% compared to the value for 3-hydroxybenzoate | Thauera aromatica | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
ATP + 3-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 4-hydroxybenzoate, with 2.3fold higher activity compared to 3-hydroxybenzoate (cf. EC 6.2.1.27) | Azoarcus sp. | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
ATP + 3-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 4-hydroxybenzoate, with 2.3fold higher activity compared to 3-hydroxybenzoate (cf. EC 6.2.1.27) | Azoarcus sp. EbN1 | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE | Azoarcus sp. |
? | x * 57500, SDS-PAGE | Thauera aromatica |
? | x * 53694, calculated from sequence | Azoarcus sp. |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxybenzoate-CoA/4-hydroxybenzoate-CoA ligase | bifunctional enzyme EC 6.2.1.27/6.2.1.37 | Thauera aromatica |
4-hydroxybenzoate-CoA ligase | - |
Azoarcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
3-hydroxybenzoate | pH and temperature not specified in the publication | Thauera aromatica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
150 | - |
3-hydroxybenzoate | pH and temperature not specified in the publication | Thauera aromatica |