Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Metallosphaera sedula |
Protein Variants | Comment | Organism |
---|---|---|
W424G | wild-type enzyme shows no activity with 4-hydroxybutanoate, mutant enzyme W424G shows activity | Metallosphaera sedula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.13 | - |
4-hydroxybutanoate | pH 7.9, 75°C, mutant enzyme W424G | Metallosphaera sedula | |
1.54 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula | |
2 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
x * 62000, calculated from sequence | Metallosphaera sedula |
64000 | - |
x * 64000, SDS-PAGE | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-hydroxybutanoate + CoA | Metallosphaera sedula | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | Metallosphaera sedula DSM 5348 | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | A4YDR9 | - |
- |
Metallosphaera sedula | A4YDT1 | - |
- |
Metallosphaera sedula | A4YGF9 | accession number for wild-type enzyme, which shows no activity with 4-hydroxybutanoate, mutant enzyme W424G shows activity | - |
Metallosphaera sedula DSM 5348 | A4YDR9 | - |
- |
Metallosphaera sedula DSM 5348 | A4YDT1 | - |
- |
Metallosphaera sedula DSM 5348 | A4YGF9 | accession number for wild-type enzyme, which shows no activity with 4-hydroxybutanoate, mutant enzyme W424G shows activity | - |
Purification (Comment) | Organism |
---|---|
- |
Metallosphaera sedula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition | cells grown under strict H2-CO2 autotrophy | Metallosphaera sedula | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.22 | - |
substrate: 4-hydroxybutanoate, pH 7.9, 75°C | Metallosphaera sedula |
1.7 | - |
substrate: 4-hydroxybutanoate, pH 7.9, 75°C | Metallosphaera sedula |
2.3 | - |
substrate: 4-hydroxybutanoate, pH 7.9, 75°C, mutant enzyme W424G | Metallosphaera sedula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-hydroxybutanoate + CoA | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle | Metallosphaera sedula | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | the enzyme has a clear preference for unsubstituted straight chain organic acids with a chain length of four or five carbons. No activity is detected with the six-carbon hexanoic acid | Metallosphaera sedula | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | tzhe enzyme is also a promiscuous acetate/propionate ligase | Metallosphaera sedula | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | wild-type enzyme shows no activity, mutant enzyme W424G shows activity | Metallosphaera sedula | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle | Metallosphaera sedula DSM 5348 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | the enzyme has a clear preference for unsubstituted straight chain organic acids with a chain length of four or five carbons. No activity is detected with the six-carbon hexanoic acid | Metallosphaera sedula DSM 5348 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | tzhe enzyme is also a promiscuous acetate/propionate ligase | Metallosphaera sedula DSM 5348 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
ATP + 4-hydroxybutanoate + CoA | wild-type enzyme shows no activity, mutant enzyme W424G shows activity | Metallosphaera sedula DSM 5348 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, SDS-PAGE | Metallosphaera sedula |
? | x * 62000, calculated from sequence | Metallosphaera sedula |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxybutyrate-CoA synthetase | - |
Metallosphaera sedula |
Msed_0394 | locus name | Metallosphaera sedula |
Msed_0406 | locus name | Metallosphaera sedula |
Msed_1353 | locus name | Metallosphaera sedula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
assay at | Metallosphaera sedula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula | |
1.8 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula | |
2.5 | - |
4-hydroxybutanoate | pH 7.9, 75°C, mutant enzyme W424G | Metallosphaera sedula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Metallosphaera sedula |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle | Metallosphaera sedula |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula | |
0.91 | - |
4-hydroxybutanoate | pH 7.9, 75°C | Metallosphaera sedula | |
2.18 | - |
4-hydroxybutanoate | pH 7.9, 75°C, mutant enzyme W424G | Metallosphaera sedula |