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Literature summary for 6.2.1.40 extracted from

  • Hawkins, A.S.; Han, Y.; Bennett, R.K.; Adams, M.W.; Kelly, R.M.
    Role of 4-hydroxybutyrate-CoA synthetase in the CO2 fixation cycle in thermoacidophilic archaea (2013), J. Biol. Chem., 288, 4012-4022.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.40

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Metallosphaera sedula

Protein Variants

Protein Variants Comment Organism
W424G wild-type enzyme shows no activity with 4-hydroxybutanoate, mutant enzyme W424G shows activity Metallosphaera sedula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13
-
 4-hydroxybutanoate pH 7.9, 75°C, mutant enzyme W424G Metallosphaera sedula
1.54
-
 4-hydroxybutanoate pH 7.9, 75°C Metallosphaera sedula
2
-
 4-hydroxybutanoate pH 7.9, 75°C Metallosphaera sedula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, calculated from sequence Metallosphaera sedula
64000
-
 x * 64000, SDS-PAGE Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-hydroxybutanoate + CoA Metallosphaera sedula the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA Metallosphaera sedula DSM 5348 the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YDR9
-
-
Metallosphaera sedula A4YDT1
-
-
Metallosphaera sedula A4YGF9 accession number for wild-type enzyme, which shows no activity with 4-hydroxybutanoate, mutant enzyme W424G shows activity
-
Metallosphaera sedula DSM 5348 A4YDR9
-
-
Metallosphaera sedula DSM 5348 A4YDT1
-
-
Metallosphaera sedula DSM 5348 A4YGF9 accession number for wild-type enzyme, which shows no activity with 4-hydroxybutanoate, mutant enzyme W424G shows activity
-

Purification (Commentary)

Purification (Comment) Organism
-
 Metallosphaera sedula

Source Tissue

Source Tissue Comment Organism Textmining
culture condition cells grown under strict H2-CO2 autotrophy Metallosphaera sedula
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.22
-
 substrate: 4-hydroxybutanoate, pH 7.9, 75°C Metallosphaera sedula
1.7
-
 substrate: 4-hydroxybutanoate, pH 7.9, 75°C Metallosphaera sedula
2.3
-
 substrate: 4-hydroxybutanoate, pH 7.9, 75°C, mutant enzyme W424G Metallosphaera sedula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-hydroxybutanoate + CoA the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle Metallosphaera sedula AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA the enzyme has a clear preference for unsubstituted straight chain organic acids with a chain length of four or five carbons. No activity is detected with the six-carbon hexanoic acid Metallosphaera sedula AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA tzhe enzyme is also a promiscuous acetate/propionate ligase Metallosphaera sedula AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA wild-type enzyme shows no activity, mutant enzyme W424G shows activity Metallosphaera sedula AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle Metallosphaera sedula DSM 5348 AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA the enzyme has a clear preference for unsubstituted straight chain organic acids with a chain length of four or five carbons. No activity is detected with the six-carbon hexanoic acid Metallosphaera sedula DSM 5348 AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA tzhe enzyme is also a promiscuous acetate/propionate ligase Metallosphaera sedula DSM 5348 AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?
ATP + 4-hydroxybutanoate + CoA wild-type enzyme shows no activity, mutant enzyme W424G shows activity Metallosphaera sedula DSM 5348 AMP + diphosphate + 4-hydroxybutanoyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Metallosphaera sedula
? x * 62000, calculated from sequence Metallosphaera sedula

Synonyms

Synonyms Comment Organism
4-hydroxybutyrate-CoA synthetase
-
 Metallosphaera sedula
Msed_0394 locus name Metallosphaera sedula
Msed_0406 locus name Metallosphaera sedula
Msed_1353 locus name Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 assay at Metallosphaera sedula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
 4-hydroxybutanoate pH 7.9, 75°C Metallosphaera sedula
1.8
-
 4-hydroxybutanoate pH 7.9, 75°C Metallosphaera sedula
2.5
-
 4-hydroxybutanoate pH 7.9, 75°C, mutant enzyme W424G Metallosphaera sedula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Metallosphaera sedula

General Information

General Information Comment Organism
physiological function the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate cycle Metallosphaera sedula

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.16
-
 4-hydroxybutanoate pH 7.9, 75°C Metallosphaera sedula
0.91
-
 4-hydroxybutanoate pH 7.9, 75°C Metallosphaera sedula
2.18
-
 4-hydroxybutanoate pH 7.9, 75°C, mutant enzyme W424G Metallosphaera sedula