Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2(DE3)/pLysS cells | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-oxocholest-4-en-26-oate + CoA | Mycobacterium tuberculosis | - |
AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA | - |
? | |
ATP + 3-oxocholest-4-en-26-oate + CoA | Mycobacterium tuberculosis H37Rv | - |
AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxy-5-cholesten-26-oate | - |
Mycobacterium tuberculosis | ? | - |
? | |
ATP + 3-hydroxy-5-cholesten-26-oate | - |
Mycobacterium tuberculosis H37Rv | ? | - |
? | |
ATP + 3-oxocholest-4-en-26-oate + CoA | - |
Mycobacterium tuberculosis | AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA | - |
? | |
ATP + 3-oxocholest-4-en-26-oate + CoA | - |
Mycobacterium tuberculosis H37Rv | AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA | - |
? | |
additional information | no activity with cholate, chenodeoxycholate, 3-oxo-23,24-bisnorchol-4-en-22-oic acid, 3-hydroxy-9-oxo-9,10-seco-23,24-bisnorchola-1,3,5(10)-trien-22-oate, 1beta(2'-propanoate)-3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-5-indanone, and 3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-1,5-indanedione | Mycobacterium tuberculosis | ? | - |
? | |
additional information | no activity with cholate, chenodeoxycholate, 3-oxo-23,24-bisnorchol-4-en-22-oic acid, 3-hydroxy-9-oxo-9,10-seco-23,24-bisnorchola-1,3,5(10)-trien-22-oate, 1beta(2'-propanoate)-3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-5-indanone, and 3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-1,5-indanedione | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fadD19 | - |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33 | - |
3-oxocholest-4-en-26-oate | at pH 7.3 and 22°C | Mycobacterium tuberculosis |