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Literature summary for 6.2.1.42 extracted from

  • Wronska, N.; Brzostek, A.; Szewczyk, R.; Sobon, A.; Dziadek, J.; Lisowska, K.
    The role of fadD19 and echA19 in sterol side chain degradation by mycobacterium smegmatis (2016), Molecules, 21, 598-611 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.42

Cloned(Commentary)

Cloned (Comment) Organism
gene fadD19, DNA and amino acid sequence determination and analysis, chromosomal localization, expression analysis Mycolicibacterium smegmatis

Protein Variants

Protein Variants Comment Organism
additional information construction of complemented mutant carrying an intact fadD19 under control of its natural putative promoter (Mycobacterium smegmatis DELTAfadD19; attB::PfadD19fadD19). The complemented strain, but not the DELTAfadD19 mutant, is able to degrade beta-sitosterol with kinetics similar to that of wild-type Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (25S)-3-oxocholest-4-en-26-oate + CoA Mycolicibacterium smegmatis
-
 AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA
-
 ?
ATP + (25S)-3-oxocholest-4-en-26-oate + CoA Mycolicibacterium smegmatis mc2155
-
 AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis I7FZL8
-
-
Mycolicibacterium smegmatis mc2155 I7FZL8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (25S)-3-oxocholest-4-en-26-oate + CoA
-
 Mycolicibacterium smegmatis AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA
-
 ?
ATP + (25S)-3-oxocholest-4-en-26-oate + CoA
-
 Mycolicibacterium smegmatis mc2155 AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
fadD19
-
 Mycolicibacterium smegmatis
steroid-CoA ligase
-
 Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycolicibacterium smegmatis

General Information

General Information Comment Organism
metabolism the enzyme plays an essential role in C-24 branched side chain sterol degradation, overview Mycolicibacterium smegmatis
physiological function the enzyme encoded by gene fadD19 plays a role in the degradation process of the side chain of cholesterol and beta-sitosterol, but is not essential for viability. FadD19 is required in the initial step of the biodegradation of C-24 branched sterol side chains in Mycobacterium smegmatis mc2155, like beta-sitosterol, but not those carrying a straight chain, like cholesterol. The enzyme has an essential role of FadD19 in the early step of beta-sitosterol degradation Mycolicibacterium smegmatis