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Literature summary for 6.2.1.43 extracted from

  • Cooke, H.A.; Zhang, J.; Griffin, M.A.; Nonaka, K.; van Lanen, S.G.; Shen, B.; Bruner, S.D.
    Characterization of NcsB2 as a promiscuous naphthoic acid/coenzyme A ligase integral to the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin (2007), J. Am. Chem. Soc., 129, 7728-7729.
    View publication on PubMed
Search for more literature for 6.2.1.43

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Streptomyces carzinostaticus
expression in Escherichia coli Streptomyces carzinostaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
 2-hydroxy-5-methyl-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.00025
-
 2-hydroxy-5-methylnaphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
0.00059
-
 2-hydroxy-7-methoxy-5-methyl-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.00059
-
 2-hydroxy-7-methoxy-5-methyl-1-naphthoate pH and temperature not specified in the publication Streptomyces carzinostaticus
0.0011
-
 2-hydroxy-5,7-dimethoxy-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.0011
-
 2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
0.0023
-
 2-hydroxy-7-methoxy-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.0023
-
 2-hydroxy-7-methoxynaphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
0.0031
-
 2,7-dihydroxy-5-methyl-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.0031
-
 2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
0.0034
-
 2-hydroxy-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.0034
-
 2-hydroxynaphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
0.018
-
 1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.018
-
 Naphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA Streptomyces carzinostaticus 100% activity AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA Streptomyces carzinostaticus the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA Streptomyces carzinostaticus ATCC 15944 100% activity AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA Streptomyces carzinostaticus ATCC 15944 the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces carzinostaticus
-
-
-
Streptomyces carzinostaticus ATCC 15944
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces carzinostaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-naphthoate + CoA kcat/Km is 0.6% compared to the wild-type value Streptomyces carzinostaticus AMP + diphosphate + 1-naphthoyl-CoA
-
 ?
ATP + 2,7-dihydroxy-5-methyl-1-naphthoate + CoA kcat/Km is 47% compared to the wild-type value Streptomyces carzinostaticus AMP + diphosphate + 2,7-dihydroxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2,7-dihydroxy-5-methyl-1-naphthoate + CoA kcat/Km is 47% compared to the wild-type value Streptomyces carzinostaticus ATCC 15944 AMP + diphosphate + 2,7-dihydroxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid + CoA 47% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate Streptomyces carzinostaticus AMP + diphosphate + ?
-
 ?
ATP + 2-hydroxy-1-naphthoate + CoA kcat/Km is 1.3% compared to the wild-type value Streptomyces carzinostaticus AMP + diphosphate + 2-hydroxy-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-1-naphthoate + CoA kcat/Km is 1.3% compared to the wild-type value Streptomyces carzinostaticus ATCC 15944 AMP + diphosphate + 2-hydroxy-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-5,7-dimethoxy-1-naphthoate + CoA kcat/Km is 35% compared to the wild-type value Streptomyces carzinostaticus AMP + diphosphate + 2-hydroxy-5,7-dimethoxy-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid + CoA 35% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate Streptomyces carzinostaticus AMP + diphosphate + ?
-
 ?
ATP + 2-hydroxy-5-methyl-1-naphthoate + CoA kcat/Km is 50% compared to the wild-type value Streptomyces carzinostaticus AMP + diphosphate + 2-hydroxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-5-methylnaphthalene-1-carboxylic acid + CoA 50% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate Streptomyces carzinostaticus AMP + diphosphate + ?
-
 ?
ATP + 2-hydroxy-7-methoxy-1-naphthoate + CoA kcat/Km is 5% compared to the wild-type value Streptomyces carzinostaticus AMP + diphosphate + 2-hydroxy-7-methoxy-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
-
 Streptomyces carzinostaticus AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA 100% activity Streptomyces carzinostaticus AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin Streptomyces carzinostaticus AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA
-
 Streptomyces carzinostaticus ATCC 15944 AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA 100% activity Streptomyces carzinostaticus ATCC 15944 AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-7-methoxy-5-methyl-1-naphthoate + CoA the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin Streptomyces carzinostaticus ATCC 15944 AMP + diphosphate + 2-hydroxy-7-methoxy-5-methyl-1-naphthoyl-CoA
-
 ?
ATP + 2-hydroxy-7-methoxynaphthalene-1-carboxylic acid + CoA 4.8% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate Streptomyces carzinostaticus AMP + diphosphate + ?
-
 ?
ATP + 2-hydroxynaphthalene-1-carboxylic acid + CoA 1.3% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate Streptomyces carzinostaticus AMP + diphosphate + ?
-
 ?
ATP + naphthalene-1-carboxylic acid + CoA 0.58% activity compared to 2-hydroxy-7-methoxy-5-methyl-1-naphthoate Streptomyces carzinostaticus AMP + diphosphate + ?
-
 ?

Synonyms

Synonyms Comment Organism
naphthoic acid/coenzyme A ligase
-
 Streptomyces carzinostaticus
naphthoyl CoA ligase
-
 Streptomyces carzinostaticus
NcsB2
-
 Streptomyces carzinostaticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.047
-
 2-hydroxy-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.05
-
 2-hydroxynaphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
0.11
-
 Naphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
0.11
-
 1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.118
-
 2-hydroxy-7-methoxy-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.12
-
 2-hydroxy-7-methoxynaphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
0.135
-
 2-hydroxy-5-methylnaphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
0.135
-
 2-hydroxy-5-methyl-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.42
-
 2-hydroxy-5,7-dimethoxynaphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
0.42
-
 2-hydroxy-5,7-dimethoxy-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
0.63
-
 2-hydroxy-7-methoxy-5-methyl-1-naphthoate pH and temperature not specified in the publication Streptomyces carzinostaticus
0.63
-
 2-hydroxy-7-methoxy-5-methyl-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
1.55
-
 2,7-dihydroxy-5-methylnaphthalene-1-carboxylic acid pH and temperature not specified in the publication Streptomyces carzinostaticus
1.55
-
 2,7-dihydroxy-5-methyl-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin Streptomyces carzinostaticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13.8
-
 2-hydroxy-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
51.3
-
 2-hydroxy-7-methoxy-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
382
-
 2-hydroxy-5,7-dimethoxy-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
500
-
 2,7-dihydroxy-5-methyl-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
540
-
 2-hydroxy-5-methyl-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus
1068
-
 2-hydroxy-7-methoxy-5-methyl-1-naphthoate pH 8.0, 24°C Streptomyces carzinostaticus