Literature summary for 6.2.1.5 extracted from

Nishimura, J.S.; Ybarra, J.; Mann, C.J.; Mitchell, T.
Sensitivity of Escherichia coli succinyl-CoA mutants at Trp beta 76 to clostripain and to trypsin. ADP and ATP protect against cleavage by clostripain at Arg beta 80 (1993), J. Biol. Chem., 268, 13717-13722.

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Protein Variants

Protein Variants	Comment	Organism
W43F/W76F/W248F	mutant W76F and mutant W43,76,248F are more sensitive to proteolysis by clostripain than the wild type enzyme or other Trp mutant proteins. Mutagenic replacement of Argbeta80, but not Argbeta14, with Lys results in an enzyme that is resistant to clostripain as wild type enzyme	Escherichia coli
W76F	mutant W76F and mutant W43,76,248F are more sensitive to proteolysis by clostripain than the wild type enzyme or other Trp mutant proteins. Mutagenic replacement of Argbeta80, but not Argbeta14, with Lys results in an enzyme that is resistant to clostripain as wild type enzyme	Escherichia coli

General Stability

General Stability	Organism
mutant W76F and mutant W43,76,248F are more sensitive to proteolysis by clostripain than the wild type enzyme or other Trp mutant proteins. ADP and ATP both protect the enzyme against inactivation by clostripain. Mutagenic replacement of Argbeta80, but not Argbeta14, with Lys results in an enzyme that is resistant to clostripain as wild type enzyme	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	mutant W76F and W43,76,248F and wild type	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + succinate + CoA	-	Escherichia coli	ADP + phosphate + succinyl-CoA	-	?

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Release 2023.1 (January 2023)