Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-O-[N-(2,3-dihydroxybenzoyl)sulfamoyl]adenosine | acts as slow-onset tight-binding inhibitor | Escherichia coli | |
5'-O-[N-(salicyl)sulfamoyl]adenosine | i.e.Sal-AMS, acts as slow-onset tight-binding inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
2,3-Dihydroxybenzoate | pH 7.8, 25°C | Escherichia coli | |
0.0029 | - |
[aryl-carrier protein EntB] | pH 7.8, 25°C | Escherichia coli | |
0.07 | - |
salicylate | pH 7.8, 25°C | Escherichia coli | |
0.07 | - |
3-hydroxybenzoate | pH 7.8, 25°C | Escherichia coli | |
0.43 | - |
ATP | pH 7.8, 25°C | Escherichia coli | |
3.1 | - |
4-Aminosalicylate | pH 7.8, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P10378 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2,3-dihydroxybenzoate + [aryl-carrier protein EntB] | - |
Escherichia coli | AMP + diphosphate + 2,3-dihydroxybenzoyl-[aryl-carrier protein EntB] | - |
? | |
ATP + 3-hydroxybenzoate + [aryl-carrier protein EntB] | - |
Escherichia coli | AMP + diphosphate + 3-hydroxybenzoyl-[aryl-carrier protein EntB] | - |
? | |
ATP + 4-aminosalicylate + [aryl-carrier protein EntB] | - |
Escherichia coli | AMP + diphosphate + 4-aminosalicyl-[aryl-carrier protein EntB] | - |
? | |
ATP + salicylate + [aryl-carrier protein EntB] | - |
Escherichia coli | AMP + diphosphate + salicyl-[aryl-carrier protein EntB] | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
3-hydroxybenzoate | pH 7.8, 25°C | Escherichia coli | |
0.8 | - |
salicylate | pH 7.8, 25°C | Escherichia coli | |
2.8 | - |
ATP | pH 7.8, 25°C | Escherichia coli | |
2.8 | - |
2,3-Dihydroxybenzoate | pH 7.8, 25°C | Escherichia coli | |
2.8 | - |
[aryl-carrier protein EntB] | pH 7.8, 25°C | Escherichia coli | |
4.4 | - |
4-Aminosalicylate | pH 7.8, 25°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000009 | - |
5'-O-[N-(salicyl)sulfamoyl]adenosine | pH 7.8, 25°C | Escherichia coli | |
0.0000038 | - |
5'-O-[N-(2,3-dihydroxybenzoyl)sulfamoyl]adenosine | pH 7.8, 25°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | EntE proceeds via a Bi Uni Uni Bi ping-pong kinetic mechanism. During the first half-reaction (adenylation), dihydroxybenzoate binds first to the free enzyme, followed by ATP and the release of diphosphate to form the adenylate intermediate. During the second half-reaction (ligation), phosphopantetheinylated EntB binds to the enzyme followed by the release of products, AMP and arylated EntB | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
3-hydroxybenzoate | pH 7.8, 25°C | Escherichia coli | |
11 | - |
salicylate | pH 7.8, 25°C | Escherichia coli | |
15 | - |
4-Aminosalicylate | pH 7.8, 25°C | Escherichia coli | |
390 | - |
ATP | pH 7.8, 25°C | Escherichia coli | |
880 | - |
2,3-Dihydroxybenzoate | pH 7.8, 25°C | Escherichia coli | |
980 | - |
[aryl-carrier protein EntB] | pH 7.8, 25°C | Escherichia coli |