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Literature summary for 6.2.1.75 extracted from
- An indoleacetate-CoA ligase and a phenylsuccinyl-CoA transferase involved in anaerobic metabolism of auxin (2016), Environ. Microbiol., 18, 3120-3132 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Aromatoleum aromaticum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (indol-3-yl)acetate | substrate inhibition | Aromatoleum aromaticum |  |
| 2,4-dichlorophenoxyacetate | - |
Aromatoleum aromaticum | |
| 2-naphthyl acetate | substrate inhibition | Aromatoleum aromaticum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
(indol-3-yl)acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
| 0.097 | - |
2-naphthyl acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
| 0.42 | - |
phenyl acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (indol-3-yl)acetate + CoA | Aromatoleum aromaticum | - |
AMP + diphosphate + (indol-3-yl)acetyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aromatoleum aromaticum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Strep-tag affinity column chromatography | Aromatoleum aromaticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (indol-3-yl)acetate + CoA | - |
Aromatoleum aromaticum | AMP + diphosphate + (indol-3-yl)acetyl-CoA | - |
? | |
| ATP + (indol-3-yl)acetate + CoA | 100% activity | Aromatoleum aromaticum | AMP + diphosphate + (indol-3-yl)acetyl-CoA | - |
? | |
| ATP + 2-methyl-indole-3-acetate + CoA | 6.3% activity compared to (indol-3-yl)acetate | Aromatoleum aromaticum | ? | - |
? | |
| ATP + 2-naphthyl acetate + CoA | 124% activity compared to (indol-3-yl)acetate | Aromatoleum aromaticum | ? | - |
? | |
| ATP + 4-hydroxyphenyl acetate + CoA | 12% activity compared to (indol-3-yl)acetate | Aromatoleum aromaticum | ? | - |
? | |
| ATP + indolepropionate + CoA | 2.9% activity compared to (indol-3-yl)acetate | Aromatoleum aromaticum | ? | - |
? | |
| ATP + phenyl acetate + CoA | 20% activity compared to (indol-3-yl)acetate | Aromatoleum aromaticum | ? | - |
? | |
| additional information | no activity with phenyl succinate, benzyl malonate, succinate, benzyl succinate, indole-3-carboxylate, indolebutyrate or L-tryptophan | Aromatoleum aromaticum | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 55000, SDS-PAGE | Aromatoleum aromaticum |
| monomer | 1 * 57000, calculated from amino acid sequence | Aromatoleum aromaticum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| iaaB | - |
Aromatoleum aromaticum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.48 | - |
phenyl acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
| 4.3 | - |
(indol-3-yl)acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
| 4.7 | - |
2-naphthyl acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.9 | - |
(indol-3-yl)acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
| 6.5 | - |
2-naphthyl acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
pH and temperature not specified in the publication | Aromatoleum aromaticum | 2,4-dichlorophenoxyacetate | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
phenyl acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
| 48 | - |
2-naphthyl acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
| 123 | - |
(indol-3-yl)acetate | pH and temperature not specified in the publication | Aromatoleum aromaticum | |
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Release 2023.1 (January 2023)
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