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Literature summary for 6.2.1.76 extracted from

  • Witkowski, A.; Thweatt, J.; Smith, S.
    Mammalian ACSF3 protein is a malonyl-CoA synthetase that supplies the chain extender units for mitochondrial fatty acid synthesis (2011), J. Biol. Chem., 286, 33729-33736 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.76

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
R354A the mutant enzyme is completely devoid of malonyl-CoA synthetase activity, although the Ala354 mutant retains the same very low activity toward acetate that is observed with the wild-type enzyme Homo sapiens
R354L the mutant enzyme is completely devoid of malonyl-CoA synthetase activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0368
-
 malonate pH 7.1, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Mus musculus 5739
-
mitochondrion human ACSF3 is located exclusively in mitochondria Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens
Mg2+ required Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64940
-
 calculated from sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + malonate + CoA Homo sapiens
-
 AMP + diphosphate + malonyl-CoA
-
 ?
ATP + malonate + CoA Mus musculus
-
 AMP + diphosphate + malonyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q4G176
-
-
Mus musculus Q3URE1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brown adipose tissue highest levels is found in brown adipose tissue, kidney, and liver Mus musculus
-
kidney highest levels is found in brown adipose tissue, kidney, and liver Mus musculus
-
liver highest levels is found in brown adipose tissue, kidney, and liver Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + malonate + CoA
-
 Homo sapiens AMP + diphosphate + malonyl-CoA
-
 ?
ATP + malonate + CoA
-
 Mus musculus AMP + diphosphate + malonyl-CoA
-
 ?
ATP + malonate + CoA no activity with oxalate, succinate, butyrate, octanoate, laurate, palmitate, oleate, or lignocerate Homo sapiens AMP + diphosphate + malonyl-CoA
-
 ?
ATP + methylmalonate + CoA 70.3% of the activity compared to malonate Homo sapiens AMP + diphosphate + methylmalonyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
ACSF3
-
 Homo sapiens
ACSF3
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.413
-
 malonate pH 7.1, 37°C Homo sapiens

General Information

General Information Comment Organism
physiological function ACSF3 is involved in the formation of the malonyl-ACP moieties required as substrates for the mitochondrial de novo fatty acid synthesis pathway Homo sapiens