Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
expression in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.529 | - |
malonate | pH 7.5, temperature not specified in the publication | Arabidopsis thaliana | |
0.66 | - |
malonate | pH 7.5, temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60062 | - |
calculated from sequence | Arabidopsis thaliana |
64130 | - |
calculated from sequence | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + malonate + CoA | Arabidopsis thaliana | - |
AMP + diphosphate + malonyl-CoA | - |
? | |
ATP + malonate + CoA | Homo sapiens | - |
AMP + diphosphate + malonyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8H151 | ecotype Columbia | - |
Homo sapiens | Q4G176 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | high expression | Homo sapiens | - |
bone | - |
Homo sapiens | - |
bone marrow | - |
Homo sapiens | - |
brain | - |
Homo sapiens | - |
connective tissue | - |
Homo sapiens | - |
eye | - |
Homo sapiens | - |
intestine | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
lung | - |
Homo sapiens | - |
lymph | high expression | Homo sapiens | - |
lymph node | high expression | Homo sapiens | - |
mammary gland | - |
Homo sapiens | - |
mouth | - |
Homo sapiens | - |
nerve | - |
Homo sapiens | - |
placenta | - |
Homo sapiens | - |
spleen | - |
Homo sapiens | - |
testis | - |
Homo sapiens | - |
thymus | high expression | Homo sapiens | - |
tonsil | high expression | Homo sapiens | - |
uterus | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-methylmalonate + CoA | activity with 2-methylmalonate is 12% compared to the activity malonate | Arabidopsis thaliana | AMP + diphosphate + 2-methylmalonyl-CoA | - |
? | |
ATP + malonate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + malonyl-CoA | - |
? | |
ATP + malonate + CoA | - |
Homo sapiens | AMP + diphosphate + malonyl-CoA | - |
? | |
ATP + malonate + CoA | little or no activity against other dicarboxylic or fatty acids | Arabidopsis thaliana | AMP + diphosphate + malonyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AAE13 | - |
Arabidopsis thaliana |
ACSF3 | - |
Homo sapiens |
Acyl Activating Enzyme13 | - |
Arabidopsis thaliana |
At3g16170 | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | calculated from sequence | - |
5.95 |
Homo sapiens | calculated from sequence | - |
8.37 |
General Information | Comment | Organism |
---|---|---|
malfunction | aae13 null mutants grow poorly and accumulated malonic and succinic acids | Arabidopsis thaliana |
physiological function | AAE13 is required to metabolize free malonate and prevent its accumulation to levels that are toxic to cell metabolism and plant growth | Arabidopsis thaliana |