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Literature summary for 6.2.1.8 extracted from

  • Foster, J.; Nakata, P.A.
    An oxalyl-CoA synthetase is important for oxalate metabolism in Saccharomyces cerevisiae (2014), FEBS Lett., 588, 160-166.
    View publication on PubMed
Search for more literature for 6.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
gene YBR222C, expression of His-tagged enzyme in Escherichia coli strain BLR(DE3) Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Saccharomyces cerevisiae
0.02
-
 oxalate pH 7.5, temperature not specified in the publication Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + oxalate + CoA Saccharomyces cerevisiae
-
 AMP + diphosphate + oxalyl-CoA
-
 ?
ATP + oxalate + CoA Saccharomyces cerevisiae BY4741
-
 AMP + diphosphate + oxalyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P38137 gene YBR222C
-
Saccharomyces cerevisiae BY4741 P38137 gene YBR222C
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography to over 90% purity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + oxalate + CoA
-
 Saccharomyces cerevisiae AMP + diphosphate + oxalyl-CoA
-
 ?
ATP + oxalate + CoA
-
 Saccharomyces cerevisiae BY4741 AMP + diphosphate + oxalyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
acyl-activating enzyme 3
-
 Saccharomyces cerevisiae
Oxalyl-CoA synthetase
-
 Saccharomyces cerevisiae
ScAAE3
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 10 activity range, profile overview Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

Expression

Organism Comment Expression
Saccharomyces cerevisiae oxalate induced the enzyme, quantitative RT-PCR reveals that ScAAE3 transcript levels are induced within min of exposure peaking at about 1 h before declining back to steady state levels up

General Information

General Information Comment Organism
malfunction a Scaae3 deletion mutant strain shows highly reduced oxalate degradation activity decreasing recovery from exposure to oxalate, e.g. by exposure to oxalate-secreting microbes, and to oxidative stress, e.g. by H2O2 Saccharomyces cerevisiae
physiological function the enzyme catalyzes the first step in a pathway of oxalate degradation to protect the cell against the harmful effects of oxalate derived from an endogenous process or an environmental source Saccharomyces cerevisiae