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Literature summary for 6.2.1.8 extracted from

  • Fan, M.; Xiao, Y.; Li, M.; Chang, W.
    Crystal structures of Arabidopsis thaliana oxalyl-CoA synthetase essential for oxalate degradation (2016), Mol. Plant, 9, 1349-1352 .
    View publication on PubMed
Search for more literature for 6.2.1.8

Crystallization (Commentary)

Crystallization (Comment) Organism
apo, ATP-bound, as well as oxalate- and AMP-bound enzyme states, hanging drop vapor diffusion method, using Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
H214A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
H319A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
K500A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
R409A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
S289A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04215
-
 oxalate pH and temperature not specified in the publication Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required in form of Mg-ATP Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + oxalate + CoA Arabidopsis thaliana oxalate is the specific substrate AMP + diphosphate + oxalyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SMT7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + oxalate + CoA oxalate is the specific substrate Arabidopsis thaliana AMP + diphosphate + oxalyl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
AAE3 acyl-activating enzyme3, gene name Arabidopsis thaliana
Oxalyl-CoA synthetase
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function enzyme-dependent degradation of oxalate is important for normal seed development and for defense against oxalate-producing fungal pathogens Arabidopsis thaliana