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Literature summary for 6.2.1.8 extracted from

  • Peng, C.; Liang, X.; Liu, E.E.; Zhang, J.J.; Peng, X.X.
    The oxalyl-CoA synthetase-regulated oxalate and its distinct effects on resistance to bacterial blight and aluminium toxicity in rice (2017), Plant Biol., 19, 345-353 .
    View publication on PubMed
Search for more literature for 6.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Oryza sativa

Protein Variants

Protein Variants Comment Organism
C219G the mutant shows 5.6% activity compared to the wild type enzyme Oryza sativa
C287G the mutant shows 25.4% activity compared to the wild type enzyme Oryza sativa
C363G the mutant shows 7.2% activity compared to the wild type enzyme Oryza sativa
C454G the mutant shows 44.5% activity compared to the wild type enzyme Oryza sativa
C473G the mutant shows 42.9% activity compared to the wild type enzyme Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide almost complete inhibition at 5 mM Oryza sativa
5,5'-dithiobis-(2-nitrobenzoic acid) less than 10% residual activity at 0.1 mM Oryza sativa
N-ethylmaleimide complete inhibition in Tris-HCl at 0.1 mM Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.73
-
 oxalate wild type enzyme, at pH 7.5 and 37°C Oryza sativa
2.62
-
 oxalate mutant enzyme C473G, at pH 7.5 and 37°C Oryza sativa
3.82
-
 oxalate mutant enzyme C219G, at pH 7.5 and 37°C Oryza sativa
4.64
-
 oxalate mutant enzyme C363G, at pH 7.5 and 37°C Oryza sativa
44.5
-
 oxalate mutant enzyme C454G, at pH 7.5 and 37°C Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Oryza sativa 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + oxalate + CoA Oryza sativa the enzyme is highly specific for oxalate AMP + diphosphate + oxalyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 cultivar Zhonghua 11
-

Purification (Commentary)

Purification (Comment) Organism
nickel chelating affinity column chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf epidermis
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + oxalate + CoA the enzyme is highly specific for oxalate Oryza sativa AMP + diphosphate + oxalyl-CoA
-
 ?
additional information no activity with malonate, succinate, malate, acetate, formate, lactate, glycolate, and glyoxylate Oryza sativa ?
-
 ?

Synonyms

Synonyms Comment Organism
AAE3
-
 Oryza sativa
OCS
-
 Oryza sativa
Oxalyl-CoA synthetase
-
 Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Oryza sativa

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Oryza sativa

General Information

General Information Comment Organism
metabolism the enzyme is involved in oxalate catabolism Oryza sativa