General Stability | Organism |
---|---|
glycerol and 2-mercaptoethanol stabilize | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Escherichia coli | |
L-Asp | - |
Escherichia coli | |
L-Asp | - |
Streptococcus equinus | |
N-ethylmaleimide | - |
Escherichia coli | |
p-mercuribenzoate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
ATP | - |
Lactiplantibacillus plantarum | |
4 | - |
NH4+ | ATP | Streptococcus equinus | |
4.2 | - |
L-Asp | - |
Lactiplantibacillus plantarum | |
26 | - |
L-Asp | - |
Streptococcus equinus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Lactiplantibacillus plantarum | |
Mg2+ | required | Escherichia coli | |
Mg2+ | required | Streptococcus equinus | |
Mg2+ | Km: 45 mM | Streptococcus equinus | |
Mn2+ | activates | Lactiplantibacillus plantarum | |
Mn2+ | Km: 3.5 mM | Lactiplantibacillus plantarum | |
Mn2+ | no activation | Escherichia coli | |
Mn2+ | no activation | Streptococcus equinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Escherichia coli | - |
overexpression of the fusion protein constructed of asparagine synthetase A structural gene is fused to the 3´end of the human carbonic anhydrase II structural gene | - |
Lactiplantibacillus plantarum | - |
- |
- |
Streptococcus equinus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Asp + hydroxylamine | - |
Escherichia coli | AMP + diphosphate + Asn + beta-aspartyl hydroxamate | - |
? | |
ATP + L-Asp + hydroxylamine | hydroxylamine is more active than NH4+ | Lactiplantibacillus plantarum | AMP + diphosphate + Asn + beta-aspartyl hydroxamate | - |
? | |
ATP + L-Asp + hydroxylamine | hydroxylamine is more active than NH4+ | Streptococcus equinus | AMP + diphosphate + Asn + beta-aspartyl hydroxamate | - |
? | |
ATP + L-Asp + NH4+ | - |
Streptococcus equinus | AMP + diphosphate + Asn | - |
? | |
ATP + L-Asp + NH4+ | no activity with GTP | Lactiplantibacillus plantarum | AMP + diphosphate + Asn | - |
? | |
ATP + L-Asp + NH4+ | no activity with ADP, CTP, UTP, or GTP | Escherichia coli | AMP + diphosphate + Asn | - |
? | |
additional information | L-Asp-dependent ATP-diphosphate exchange in the absence of added NH4+ | Escherichia coli | ? | - |
? | |
additional information | no activity with glutamine | Lactiplantibacillus plantarum | ? | - |
? | |
additional information | no activity with glutamine | Escherichia coli | ? | - |
? | |
additional information | no activity with glutamine | Streptococcus equinus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | 42 | - |
Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Lactiplantibacillus plantarum |
8 | 8.4 | - |
Escherichia coli |