Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a dph6 gene knockout mutant strain | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + diphthine-[translation elongation factor 2] + NH3 | Saccharomyces cerevisiae | - |
AMP + diphosphate + diphthamide-[translation elongation factor 2] | - |
? | |
ATP + diphthine-[translation elongation factor 2] + NH3 | Saccharomyces cerevisiae ATCC 204508 / S288c | - |
AMP + diphosphate + diphthamide-[translation elongation factor 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12429 | - |
- |
Saccharomyces cerevisiae ATCC 204508 / S288c | Q12429 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + diphthine-[translation elongation factor 2] + NH3 | - |
Saccharomyces cerevisiae | AMP + diphosphate + diphthamide-[translation elongation factor 2] | - |
? | |
ATP + diphthine-[translation elongation factor 2] + NH3 | - |
Saccharomyces cerevisiae ATCC 204508 / S288c | AMP + diphosphate + diphthamide-[translation elongation factor 2] | - |
? | |
additional information | the repurified DELTAdph7 eEF-2 is a substrate for the amidation by Dph6 alone. In contrast, FLAG-tagged DELTAdph7 eEF-2 incubated without Dph7 does not form diphthamide | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the repurified DELTAdph7 eEF-2 is a substrate for the amidation by Dph6 alone. In contrast, FLAG-tagged DELTAdph7 eEF-2 incubated without Dph7 does not form diphthamide | Saccharomyces cerevisiae ATCC 204508 / S288c | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
diphthamide synthetase | - |
Saccharomyces cerevisiae |
DPH6 | - |
Saccharomyces cerevisiae |
YLR143w | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | diphthamide is formed on eEF-2 only in the presence of both Dph6 and Dph7 enzymes. Enzyme DPH6 catalyzes the last step in the diphthamide synthesis pathway, the amidation of diphthine bound to translation elongation factor 2, while enzyme DPH7 is a methylesterase converting methylated diphthine to diphthine, so that Dph6 can convert it to diphthamide. Diphthamide synthesis pathway, overview | Saccharomyces cerevisiae |