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Literature summary for 6.3.1.14 extracted from

  • Lin, Z.; Su, X.; Chen, W.; Ci, B.; Zhang, S.; Lin, H.
    Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesis (2014), J. Am. Chem. Soc., 136, 6179-6182 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of a dph6 gene knockout mutant strain Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + diphthine-[translation elongation factor 2] + NH3 Saccharomyces cerevisiae
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AMP + diphosphate + diphthamide-[translation elongation factor 2]
-
?
ATP + diphthine-[translation elongation factor 2] + NH3 Saccharomyces cerevisiae ATCC 204508 / S288c
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AMP + diphosphate + diphthamide-[translation elongation factor 2]
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12429
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Saccharomyces cerevisiae ATCC 204508 / S288c Q12429
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + diphthine-[translation elongation factor 2] + NH3
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Saccharomyces cerevisiae AMP + diphosphate + diphthamide-[translation elongation factor 2]
-
?
ATP + diphthine-[translation elongation factor 2] + NH3
-
Saccharomyces cerevisiae ATCC 204508 / S288c AMP + diphosphate + diphthamide-[translation elongation factor 2]
-
?
additional information the repurified DELTAdph7 eEF-2 is a substrate for the amidation by Dph6 alone. In contrast, FLAG-tagged DELTAdph7 eEF-2 incubated without Dph7 does not form diphthamide Saccharomyces cerevisiae ?
-
?
additional information the repurified DELTAdph7 eEF-2 is a substrate for the amidation by Dph6 alone. In contrast, FLAG-tagged DELTAdph7 eEF-2 incubated without Dph7 does not form diphthamide Saccharomyces cerevisiae ATCC 204508 / S288c ?
-
?

Synonyms

Synonyms Comment Organism
diphthamide synthetase
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Saccharomyces cerevisiae
DPH6
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Saccharomyces cerevisiae
YLR143w
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Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism diphthamide is formed on eEF-2 only in the presence of both Dph6 and Dph7 enzymes. Enzyme DPH6 catalyzes the last step in the diphthamide synthesis pathway, the amidation of diphthine bound to translation elongation factor 2, while enzyme DPH7 is a methylesterase converting methylated diphthine to diphthine, so that Dph6 can convert it to diphthamide. Diphthamide synthesis pathway, overview Saccharomyces cerevisiae