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Literature summary for 6.3.1.2 extracted from

  • Shin, D.; Park, C.
    N-terminal extension of canine glutamine synthetase created by splicing alters its enzymatic property (2004), J. Biol. Chem., 279, 1184-1190.
    View publication on PubMed
Search for more literature for 6.3.1.2

Cloned(Commentary)

Cloned (Comment) Organism
short and long isoform expressed in Escherichia coli BL21(DE3) Canis lupus familiaris

Inhibitors

Inhibitors Comment Organism Structure
L-methionine-S-sulfoximine
-
 Canis lupus familiaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 L-glutamate short isoform, pH 7.2, 37°C Canis lupus familiaris
1.3
-
 L-glutamate long isoform, pH 7.2, 37°C Canis lupus familiaris
1.3
-
 ATP short isoform, pH 7.2, 37°C Canis lupus familiaris
1.9
-
 ATP long isoform, pH 7.2, 37°C Canis lupus familiaris

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Canis lupus familiaris 5829
-
membrane
-
 Canis lupus familiaris 16020
-
nucleus low activity Canis lupus familiaris 5634
-
particle-bound low activity Canis lupus familiaris
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE, short isoform Canis lupus familiaris
49000
-
 SDS-PAGE, long isoform Canis lupus familiaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glutamate + NH4+ Mus musculus central enzyme of nitrogen metabolism, elimination of glutamate from animal brain ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+ Homo sapiens central enzyme of nitrogen metabolism, elimination of glutamate from animal brain ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+ Canis lupus familiaris central enzyme of nitrogen metabolism, elimination of glutamate from animal brain ADP + phosphate + L-glutamine
-
 ?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Canis lupus familiaris
-
 two isoforms generated by alternative splicing, differences in molecular weights
-
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins using His-tag Canis lupus familiaris

Source Tissue

Source Tissue Comment Organism Textmining
brain short and long isoform Canis lupus familiaris
-
liver short and long isoform Canis lupus familiaris
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glutamate + NH4+
-
 Mus musculus ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+
-
 Homo sapiens ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+
-
 Canis lupus familiaris ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, elimination of glutamate from animal brain Mus musculus ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, elimination of glutamate from animal brain Homo sapiens ADP + phosphate + L-glutamine
-
 ?
ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, elimination of glutamate from animal brain Canis lupus familiaris ADP + phosphate + L-glutamine
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.067
-
 L-methionine-S-sulfoximine short isoform, pH 7.2, 37°C Canis lupus familiaris
0.124
-
 L-methionine-S-sulfoximine long isoform, pH 7.2, 37°C Canis lupus familiaris