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Literature summary for 6.3.1.2 extracted from
- Purification and properties of a cytosolic glutamine synthetase expressed in Nicotiana plumbaginifolia cultured cells (2000), Plant Physiol. Biochem., 38, 201-207.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
NH4+ | pH 7.4, 35°C | Nicotiana plumbaginifolia |  |
| 0.79 | - |
ATP | pH 7.4, 35°C | Nicotiana plumbaginifolia | |
| 5.71 | - |
glutamate | pH 7.4, 35°C | Nicotiana plumbaginifolia | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Nicotiana plumbaginifolia | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37400 | - |
SDS-PAGE | Nicotiana plumbaginifolia |
| 305000 | - |
native PAGE | Nicotiana plumbaginifolia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glutamate + NH4+ | Nicotiana plumbaginifolia | central enzyme of nitrogen metabolism, ammonia assimilation | ADP + phosphate + L-glutamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana plumbaginifolia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Nicotiana plumbaginifolia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | leaf protoplast derived suspension culture | Nicotiana plumbaginifolia | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glutamate + NH4+ | - |
Nicotiana plumbaginifolia | ADP + phosphate + L-glutamine | - |
? | |
| ATP + glutamate + NH4+ | central enzyme of nitrogen metabolism, ammonia assimilation | Nicotiana plumbaginifolia | ADP + phosphate + L-glutamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 37400, subunit mass by SDS-PAGE, native mass by native PAGE | Nicotiana plumbaginifolia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Nicotiana plumbaginifolia |
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Release 2023.1 (January 2023)
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