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Literature summary for 6.3.1.2 extracted from

  • Harth, G.; a-Gali, S.M.; Tullius, M.V.; Horwitz, M.A.
    All four Mycobacterium tuberculosis glnA genes encode glutamine synthetase activities but only GlnA1 is abundantly expressed and essential for bacterial homeostasis (2005), Mol. Microbiol., 58, 1157-1172.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene glnA1, i.e. gene Rv2220, DNA and amino acid sequence determination and analysis, isozyme expression analysis. Except for glnA1, the genes are all located in a multigene operon, which also contains the four genes encoding the regulating enzymes of the pathway, overview. Overexpression of the glnA1-gln E-glnA2 cluster in Mycobacterium smegmatis as functional multimeric enzymes Mycobacterium tuberculosis
gene glnA2, i.e. gene Rv2222c, DNA and amino acid sequence determination and analysis, isozyme expression analysis. Except for glnA1, the genes are all located in a multigene operon, which also contains the four genes encoding the regulating enzymes of the pathway, overview. Overexpression of the glnA1-gln E-glnA2 cluster in Mycobacterium smegmatis as functional multimeric enzymes Mycobacterium tuberculosis
gene glnA3, i.e. gene Rv1878, DNA and amino acid sequence determination and analysis, isozyme expression analysis. Except for glnA1, the genes are all located in a multigene operon, which also contains the four genes encoding the regulating enzymes of the pathway, overview. Overexpression of the glnA1-gln E-glnA2 cluster in Mycobacterium smegmatis as functional multimeric enzymes Mycobacterium tuberculosis
gene glnA4, i.e. gene Rv2860c, DNA and amino acid sequence determination and analysis, isozyme expression analysis. Except for glnA1, the genes are all located in a multigene operon, which also contains the four genes encoding the regulating enzymes of the pathway, overview. Overexpression of the glnA1-gln E-glnA2 cluster in Mycobacterium smegmatis as functional multimeric enzymes Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
L-methionine-(S,R)-sulfoximine
-
Mycobacterium tuberculosis
L-methionine-(S,R)-sulphoximine
-
Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46700
-
x * 46700, about, GlnA3, sequence calculation Mycobacterium tuberculosis
49600
-
x * 49600, about, GlnA2, sequence calculation Mycobacterium tuberculosis
49700
-
x * 49700, about, GlnA4, sequence calculation Mycobacterium tuberculosis
53600
-
x * 53600, about, GlnA1, sequence calculation Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ATP + 2 L-glutamate + 2 NH3 Mycobacterium tuberculosis GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine and is essential for bacterial growth 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine
-
?
2 ATP + 2 L-glutamate + 2 NH3 Mycobacterium tuberculosis H37Rv GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine and is essential for bacterial growth 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine
-
?
ATP + L-glutamate + NH3 Mycobacterium tuberculosis GlnA1 catalyses the synthesis of L-glutamine and is nonessential for bacterial growth ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 Mycobacterium tuberculosis GlnA3 catalyses the synthesis of L-glutamine and are nonessential for bacterial growth ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 Mycobacterium tuberculosis GlnA4 catalyses the synthesis of L-glutamine and are nonessential for bacterial growth ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 Mycobacterium tuberculosis H37Rv GlnA1 catalyses the synthesis of L-glutamine and is nonessential for bacterial growth ADP + phosphate + L-glutamine
-
?
additional information Mycobacterium tuberculosis glutamine synthetase is involved in nitrogen metabolism, and four enzymes are regulating glutamine synthetase activity and/or nitrogen and glutamate metabolism: adenylyl transferase, i.e. GlnE, gamma-glutamylcysteine synthase, i.e. GshA, UDP-N-acetylmuramoylalanine-D-glutamate ligase, i.e. MurD, and glutamate racemase, i.e. MurI ?
-
?
additional information Mycobacterium tuberculosis H37Rv glutamine synthetase is involved in nitrogen metabolism, and four enzymes are regulating glutamine synthetase activity and/or nitrogen and glutamate metabolism: adenylyl transferase, i.e. GlnE, gamma-glutamylcysteine synthase, i.e. GshA, UDP-N-acetylmuramoylalanine-D-glutamate ligase, i.e. MurD, and glutamate racemase, i.e. MurI ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis O07752 gene glnA3 or gene Rv1878, encoding isozyme GlnA3
-
Mycobacterium tuberculosis O33342 gene glnA4 or gene Rv2860c, encoding isozyme GlnA4
-
Mycobacterium tuberculosis P9WN39 gene glnA1 or gene Rv2220 encoding isozyme GlnA1
-
Mycobacterium tuberculosis P9WN39 gene glnA2 or gene Rv2222c encoding isozyme GlnA2
-
Mycobacterium tuberculosis H37Rv P9WN39 gene glnA1 or gene Rv2220 encoding isozyme GlnA1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + 2 L-glutamate + 2 NH3 GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine and is essential for bacterial growth Mycobacterium tuberculosis 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine
-
?
2 ATP + 2 L-glutamate + 2 NH3 GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine Mycobacterium tuberculosis 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine
-
?
2 ATP + 2 L-glutamate + 2 NH3 GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine and is essential for bacterial growth Mycobacterium tuberculosis H37Rv 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine
-
?
2 ATP + 2 L-glutamate + 2 NH3 GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine Mycobacterium tuberculosis H37Rv 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine
-
?
ATP + L-glutamate + NH3 GlnA1 catalyses the synthesis of L-glutamine and is nonessential for bacterial growth Mycobacterium tuberculosis ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 GlnA3 catalyses the synthesis of L-glutamine and are nonessential for bacterial growth Mycobacterium tuberculosis ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 GlnA4 catalyses the synthesis of L-glutamine and are nonessential for bacterial growth Mycobacterium tuberculosis ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 GlnA1 catalyses the synthesis of L-glutamine Mycobacterium tuberculosis ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 GlnA3 catalyses the synthesis of L-glutamine Mycobacterium tuberculosis ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 GlnA4 catalyses the synthesis of L-glutamine Mycobacterium tuberculosis ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 GlnA1 catalyses the synthesis of L-glutamine and is nonessential for bacterial growth Mycobacterium tuberculosis H37Rv ADP + phosphate + L-glutamine
-
?
ATP + L-glutamate + NH3 GlnA1 catalyses the synthesis of L-glutamine Mycobacterium tuberculosis H37Rv ADP + phosphate + L-glutamine
-
?
additional information glutamine synthetase is involved in nitrogen metabolism, and four enzymes are regulating glutamine synthetase activity and/or nitrogen and glutamate metabolism: adenylyl transferase, i.e. GlnE, gamma-glutamylcysteine synthase, i.e. GshA, UDP-N-acetylmuramoylalanine-D-glutamate ligase, i.e. MurD, and glutamate racemase, i.e. MurI Mycobacterium tuberculosis ?
-
?
additional information glutamine synthetase is involved in nitrogen metabolism, and four enzymes are regulating glutamine synthetase activity and/or nitrogen and glutamate metabolism: adenylyl transferase, i.e. GlnE, gamma-glutamylcysteine synthase, i.e. GshA, UDP-N-acetylmuramoylalanine-D-glutamate ligase, i.e. MurD, and glutamate racemase, i.e. MurI Mycobacterium tuberculosis H37Rv ?
-
?

Subunits

Subunits Comment Organism
? x * 46700, about, GlnA3, sequence calculation Mycobacterium tuberculosis
? x * 49600, about, GlnA2, sequence calculation Mycobacterium tuberculosis
? x * 49700, about, GlnA4, sequence calculation Mycobacterium tuberculosis
? x * 53600, about, GlnA1, sequence calculation Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
GlnA1
-
Mycobacterium tuberculosis
GlnA2
-
Mycobacterium tuberculosis
GlnA3
-
Mycobacterium tuberculosis
GlnA4
-
Mycobacterium tuberculosis
Glutamine synthetase
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mycobacterium tuberculosis