Cloned (Comment) | Organism |
---|---|
gene glnA1, i.e. gene Rv2220, DNA and amino acid sequence determination and analysis, isozyme expression analysis. Except for glnA1, the genes are all located in a multigene operon, which also contains the four genes encoding the regulating enzymes of the pathway, overview. Overexpression of the glnA1-gln E-glnA2 cluster in Mycobacterium smegmatis as functional multimeric enzymes | Mycobacterium tuberculosis |
gene glnA2, i.e. gene Rv2222c, DNA and amino acid sequence determination and analysis, isozyme expression analysis. Except for glnA1, the genes are all located in a multigene operon, which also contains the four genes encoding the regulating enzymes of the pathway, overview. Overexpression of the glnA1-gln E-glnA2 cluster in Mycobacterium smegmatis as functional multimeric enzymes | Mycobacterium tuberculosis |
gene glnA3, i.e. gene Rv1878, DNA and amino acid sequence determination and analysis, isozyme expression analysis. Except for glnA1, the genes are all located in a multigene operon, which also contains the four genes encoding the regulating enzymes of the pathway, overview. Overexpression of the glnA1-gln E-glnA2 cluster in Mycobacterium smegmatis as functional multimeric enzymes | Mycobacterium tuberculosis |
gene glnA4, i.e. gene Rv2860c, DNA and amino acid sequence determination and analysis, isozyme expression analysis. Except for glnA1, the genes are all located in a multigene operon, which also contains the four genes encoding the regulating enzymes of the pathway, overview. Overexpression of the glnA1-gln E-glnA2 cluster in Mycobacterium smegmatis as functional multimeric enzymes | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-methionine-(S,R)-sulfoximine | - |
Mycobacterium tuberculosis | |
L-methionine-(S,R)-sulphoximine | - |
Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46700 | - |
x * 46700, about, GlnA3, sequence calculation | Mycobacterium tuberculosis |
49600 | - |
x * 49600, about, GlnA2, sequence calculation | Mycobacterium tuberculosis |
49700 | - |
x * 49700, about, GlnA4, sequence calculation | Mycobacterium tuberculosis |
53600 | - |
x * 53600, about, GlnA1, sequence calculation | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + 2 L-glutamate + 2 NH3 | Mycobacterium tuberculosis | GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine and is essential for bacterial growth | 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine | - |
? | |
2 ATP + 2 L-glutamate + 2 NH3 | Mycobacterium tuberculosis H37Rv | GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine and is essential for bacterial growth | 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine | - |
? | |
ATP + L-glutamate + NH3 | Mycobacterium tuberculosis | GlnA1 catalyses the synthesis of L-glutamine and is nonessential for bacterial growth | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | Mycobacterium tuberculosis | GlnA3 catalyses the synthesis of L-glutamine and are nonessential for bacterial growth | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | Mycobacterium tuberculosis | GlnA4 catalyses the synthesis of L-glutamine and are nonessential for bacterial growth | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | Mycobacterium tuberculosis H37Rv | GlnA1 catalyses the synthesis of L-glutamine and is nonessential for bacterial growth | ADP + phosphate + L-glutamine | - |
? | |
additional information | Mycobacterium tuberculosis | glutamine synthetase is involved in nitrogen metabolism, and four enzymes are regulating glutamine synthetase activity and/or nitrogen and glutamate metabolism: adenylyl transferase, i.e. GlnE, gamma-glutamylcysteine synthase, i.e. GshA, UDP-N-acetylmuramoylalanine-D-glutamate ligase, i.e. MurD, and glutamate racemase, i.e. MurI | ? | - |
? | |
additional information | Mycobacterium tuberculosis H37Rv | glutamine synthetase is involved in nitrogen metabolism, and four enzymes are regulating glutamine synthetase activity and/or nitrogen and glutamate metabolism: adenylyl transferase, i.e. GlnE, gamma-glutamylcysteine synthase, i.e. GshA, UDP-N-acetylmuramoylalanine-D-glutamate ligase, i.e. MurD, and glutamate racemase, i.e. MurI | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | O07752 | gene glnA3 or gene Rv1878, encoding isozyme GlnA3 | - |
Mycobacterium tuberculosis | O33342 | gene glnA4 or gene Rv2860c, encoding isozyme GlnA4 | - |
Mycobacterium tuberculosis | P9WN39 | gene glnA1 or gene Rv2220 encoding isozyme GlnA1 | - |
Mycobacterium tuberculosis | P9WN39 | gene glnA2 or gene Rv2222c encoding isozyme GlnA2 | - |
Mycobacterium tuberculosis H37Rv | P9WN39 | gene glnA1 or gene Rv2220 encoding isozyme GlnA1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + 2 L-glutamate + 2 NH3 | GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine and is essential for bacterial growth | Mycobacterium tuberculosis | 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine | - |
? | |
2 ATP + 2 L-glutamate + 2 NH3 | GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine | Mycobacterium tuberculosis | 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine | - |
? | |
2 ATP + 2 L-glutamate + 2 NH3 | GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine and is essential for bacterial growth | Mycobacterium tuberculosis H37Rv | 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine | - |
? | |
2 ATP + 2 L-glutamate + 2 NH3 | GlnA2 catalyses the synthesis of D-glutamine and D-isoglutamine | Mycobacterium tuberculosis H37Rv | 2 ADP + 2 phosphate + D-glutamine + D-isoglutamine | - |
? | |
ATP + L-glutamate + NH3 | GlnA1 catalyses the synthesis of L-glutamine and is nonessential for bacterial growth | Mycobacterium tuberculosis | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | GlnA3 catalyses the synthesis of L-glutamine and are nonessential for bacterial growth | Mycobacterium tuberculosis | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | GlnA4 catalyses the synthesis of L-glutamine and are nonessential for bacterial growth | Mycobacterium tuberculosis | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | GlnA1 catalyses the synthesis of L-glutamine | Mycobacterium tuberculosis | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | GlnA3 catalyses the synthesis of L-glutamine | Mycobacterium tuberculosis | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | GlnA4 catalyses the synthesis of L-glutamine | Mycobacterium tuberculosis | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | GlnA1 catalyses the synthesis of L-glutamine and is nonessential for bacterial growth | Mycobacterium tuberculosis H37Rv | ADP + phosphate + L-glutamine | - |
? | |
ATP + L-glutamate + NH3 | GlnA1 catalyses the synthesis of L-glutamine | Mycobacterium tuberculosis H37Rv | ADP + phosphate + L-glutamine | - |
? | |
additional information | glutamine synthetase is involved in nitrogen metabolism, and four enzymes are regulating glutamine synthetase activity and/or nitrogen and glutamate metabolism: adenylyl transferase, i.e. GlnE, gamma-glutamylcysteine synthase, i.e. GshA, UDP-N-acetylmuramoylalanine-D-glutamate ligase, i.e. MurD, and glutamate racemase, i.e. MurI | Mycobacterium tuberculosis | ? | - |
? | |
additional information | glutamine synthetase is involved in nitrogen metabolism, and four enzymes are regulating glutamine synthetase activity and/or nitrogen and glutamate metabolism: adenylyl transferase, i.e. GlnE, gamma-glutamylcysteine synthase, i.e. GshA, UDP-N-acetylmuramoylalanine-D-glutamate ligase, i.e. MurD, and glutamate racemase, i.e. MurI | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46700, about, GlnA3, sequence calculation | Mycobacterium tuberculosis |
? | x * 49600, about, GlnA2, sequence calculation | Mycobacterium tuberculosis |
? | x * 49700, about, GlnA4, sequence calculation | Mycobacterium tuberculosis |
? | x * 53600, about, GlnA1, sequence calculation | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
GlnA1 | - |
Mycobacterium tuberculosis |
GlnA2 | - |
Mycobacterium tuberculosis |
GlnA3 | - |
Mycobacterium tuberculosis |
GlnA4 | - |
Mycobacterium tuberculosis |
Glutamine synthetase | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |