Protein Variants | Comment | Organism |
---|---|---|
A305G | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 16% of wild-type activity with L-glutamate, 2% of wild-type activity with L-glutamine | Bacillus subtilis |
E304D | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 37% of wild-type activity with L-glutamate, 8% of wild-type activity with L-glutamine | Bacillus subtilis |
E304H | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 43% of wild-type activity with L-glutamate, 5% of wild-type activity with L-glutamine | Bacillus subtilis |
G302A | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 80% of wild-type activity with L-glutamate, 58% of wild-type activity with L-glutamine | Bacillus subtilis |
P306A | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 160% of wild-type activity with L-glutamate, 130% of wild-type activity with L-glutamine | Bacillus subtilis |
V300A | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 76% of wild-type activity with L-glutamate, 18% of wild-type activity with L-glutamine | Bacillus subtilis |
Y303A | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 36% of wild-type activity with L-glutamate, 8% of wild-type activity with L-glutamine | Bacillus subtilis |
Y303H | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 150% of wild-type activity with L-glutamate, 100% of wild-type activity with L-glutamine | Bacillus subtilis |
Y303L | mutation in the E304 flap, a flexible 7-residue loop over the entrance to the active site. 87% of wild-type activity with L-glutamate, 46% of wild-type activity with L-glutamine | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Bacillus subtilis | |
glycine | - |
Bacillus subtilis | |
L-glutamine | feedback inhibition | Bacillus subtilis | |
L-methionine-S-sulfoximine | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
NH3 | wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | |
0.68 | - |
hydroxylamine | mutant E304H, 25°C, pH not specified in the publication | Bacillus subtilis | |
0.74 | - |
hydroxylamine | mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | |
0.83 | - |
hydroxylamine | wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | |
1.2 | - |
ATP | mutant E304H, 25°C, pH not specified in the publication | Bacillus subtilis | |
2.4 | - |
ATP | wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | |
3.4 | - |
L-glutamate | mutant E304H, 25°C, pH not specified in the publication | Bacillus subtilis | |
5.3 | - |
ATP | mutant G302A, 25°C, pH not specified in the publication | Bacillus subtilis | |
9.4 | - |
ATP | mutant Y303H, 25°C, pH not specified in the publication | Bacillus subtilis | |
10 | - |
L-glutamate | mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | |
11 | - |
ATP | mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | |
27 | - |
L-glutamate | wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | |
32 | - |
NH3 | mutant E304H, 25°C, pH not specified in the publication | Bacillus subtilis | |
59 | - |
L-glutamate | mutant Y303H, 25°C, pH not specified in the publication | Bacillus subtilis | |
84 | - |
L-glutamate | mutant G302A, 25°C, pH not specified in the publication | Bacillus subtilis | |
120 | - |
NH3 | mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH3 | - |
Bacillus subtilis | ADP + phosphate + L-glutamine | - |
? | |
L-glutamine + hydroxylamine + ADP | partial reverse reaction | Bacillus subtilis | gamma-glutamylhydroxamate + NH3 + ? | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.1 | - |
mutant Y303H, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
0.1 | - |
mutant Y303L, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
0.12 | - |
mutant V300A, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
0.13 | - |
wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
0.2 | - |
mutant P306A, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
0.25 | - |
mutant P306A, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
0.28 | - |
mutant G302A, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
0.5 | - |
wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
0.61 | - |
mutant Y303A, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
1 | - |
mutant Y303H, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
1.3 | - |
mutant Y303A, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
1.4 | - |
mutant V300A, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
1.5 | - |
mutant P306A, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
1.5 | - |
mutant Y303L, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
1.7 | - |
mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
1.7 | - |
mutant G302A, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
2.1 | - |
mutant A305G, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
2.4 | - |
wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
2.7 | - |
mutant A305G, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
4.1 | - |
mutant Y303H, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
7 | - |
mutant V300A, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
11 | - |
mutant G302A, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
13 | - |
mutant Y303A, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
19 | - |
mutant Y303L, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
20 | - |
mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
26 | - |
mutant E304A, 25°C, pH not specified in the publication | Bacillus subtilis | L-methionine-S-sulfoximine | |
30 | - |
mutant E304A, 25°C, pH not specified in the publication | Bacillus subtilis | AMP | |
31 | - |
wild-type, 25°C, pH not specified in the publication | Bacillus subtilis | glycine | |
79 | - |
mutant E304D, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
140 | - |
mutant A305G, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
140 | - |
mutant E304A, 25°C, pH not specified in the publication | Bacillus subtilis | L-glutamine | |
350 | - |
mutant A305G, 25°C, pH not specified in the publication | Bacillus subtilis | glycine |