Application | Comment | Organism |
---|---|---|
synthesis | high efficiency L-Gln production by coupling genetically engineered Bacillus subtilis glutamine synthetase with yeast alcoholic fermentation system using small ubiquitin-related modifier fusion technology SUMO and 0.1% lactose as inducer. Fusion protein is expressed in totally soluble form in Escherichia coli and can be purified to 90% purity by nickel nitrilo-triacetic acid resin chromatography with a yield of 625 mg per liter fermentation culture. About 121 mg recombinant GS is obtained from 1 l fermentation culture with 96% purity and 23 U/mg activity | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
coupling genetically engineered Bacillus subtilis glutamine synthetase with yeast alcoholic fermentation system using small ubiquitin-related modifier fusion technology SUMO | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, fusion-protein with SUMO-tag, SDS-PAGE and calculated | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P12425 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23 | - |
pH 6.5, 37°C, presence of Mn2+ | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + NH4+ | - |
Bacillus subtilis | ADP + phosphate + L-glutamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, fusion-protein with SUMO-tag, SDS-PAGE and calculated | Bacillus subtilis |