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Literature summary for 6.3.1.2 extracted from

  • Wang, Q.; Min, C.; Yan, T.; Pu, H.; Xin, Y.; Zhang, S.; Luo, L.; Yin, Z.
    Production of glutamine synthetase in Escherichia coli using SUMO fusion partner and application to L-glutamine synthesis (2011), World J. Microbiol. Biotechnol., 27, 2603-2610.
    View publication on PubMed

Application

Application Comment Organism
synthesis high efficiency L-Gln production by coupling genetically engineered Bacillus subtilis glutamine synthetase with yeast alcoholic fermentation system using small ubiquitin-related modifier fusion technology SUMO and 0.1% lactose as inducer. Fusion protein is expressed in totally soluble form in Escherichia coli and can be purified to 90% purity by nickel nitrilo-triacetic acid resin chromatography with a yield of 625 mg per liter fermentation culture. About 121 mg recombinant GS is obtained from 1 l fermentation culture with 96% purity and 23 U/mg activity Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
coupling genetically engineered Bacillus subtilis glutamine synthetase with yeast alcoholic fermentation system using small ubiquitin-related modifier fusion technology SUMO Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, fusion-protein with SUMO-tag, SDS-PAGE and calculated Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P12425
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23
-
pH 6.5, 37°C, presence of Mn2+ Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamate + NH4+
-
Bacillus subtilis ADP + phosphate + L-glutamine
-
?

Subunits

Subunits Comment Organism
? x * 70000, fusion-protein with SUMO-tag, SDS-PAGE and calculated Bacillus subtilis