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Literature summary for 6.3.1.2 extracted from
- beta-Glutamate as a substrate for glutamine synthetase (2001), Appl. Environ. Microbiol., 67, 4458-4463.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Archaeoglobus fulgidus |
| overexpression in Escherichia coli | Methanocaldococcus jannaschii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6 | - |
ATP | pH 7.0, 37°C | Methanohalophilus portucalensis |  |
| 58 | - |
L-glutamate | pH 7.0, 60°C | Methanocaldococcus jannaschii | |
| 104 | - |
L-glutamate | pH 7.0, 37°C | Methanohalophilus portucalensis | |
| 175 | - |
3-aminopentanedioate | pH 7.0, 37°C | Methanohalophilus portucalensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | assay in presence of 55 mM Mg2+ | Methanohalophilus portucalensis | |
| Mg2+ | assay in presence of 55 mM Mg2+ | Archaeoglobus fulgidus | |
| Mg2+ | assay in presence of 55 mM Mg2+ | Methanocaldococcus jannaschii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | O29313 | - |
- |
| Archaeoglobus fulgidus VC-16 | O29313 | - |
- |
| Methanocaldococcus jannaschii | Q60182 | - |
- |
| Methanohalophilus portucalensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Archaeoglobus fulgidus |
- |
Methanocaldococcus jannaschii |
| partial | Methanohalophilus portucalensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
pH 7.0, 37°C | Methanohalophilus portucalensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-aminopentanedioate + hydroxylamine | the activity with is 3-aminopentanedioate (beta-glutamate) is 7fold less than the rate obtained with alpha-glutamate | Methanohalophilus portucalensis | ADP + phosphate + ? | - |
? | |
| ATP + 3-aminopentanedioate + hydroxylamine | the enzyme is more selective for L-glutamate (alpha-glutamate) than for 3-aminopentanedioate (beta-glutamate) as a substrate | Archaeoglobus fulgidus | ADP + phosphate + ? | - |
? | |
| ATP + 3-aminopentanedioate + hydroxylamine | the enzyme is more selective for L-glutamate (alpha-glutamate) than for 3-aminopentanedioate (beta-glutamate) as a substrate | Archaeoglobus fulgidus VC-16 | ADP + phosphate + ? | - |
? | |
| ATP + 3-aminopentanedioate + hydroxylamine | the enzyme is more selective for L-glutamate (alpha-glutamate) than 3-aminopentanedioate (beta-glutamate) as a substrate | Methanocaldococcus jannaschii | ADP + phosphate + gamma-glutamylhydroxamate | - |
? | |
| ATP + L-glutamate + hydroxylamine | the activity with L-glutamate is 7fold higher than the rate obtained with 3-aminopentanedioate (beta-glutamate) | Methanohalophilus portucalensis | ADP + phosphate + gamma-L-glutamylhydroxamate | - |
? | |
| ATP + L-glutamate + hydroxylamine | the enzyme is more selective for L-glutamate (alpha-glutamate) than for 3-aminopentanedioate (beta-glutamate) as a substrate | Archaeoglobus fulgidus | ADP + phosphate + gamma-L-glutamylhydroxamate | - |
? | |
| ATP + L-glutamate + hydroxylamine | the enzyme is more selective for L-glutamate (alpha-glutamate) than for 3-aminopentanedioate (beta-glutamate) as a substrate | Methanocaldococcus jannaschii | ADP + phosphate + gamma-L-glutamylhydroxamate | - |
? | |
| ATP + L-glutamate + hydroxylamine | the enzyme is more selective for L-glutamate (alpha-glutamate) than for 3-aminopentanedioate (beta-glutamate) as a substrate | Archaeoglobus fulgidus VC-16 | ADP + phosphate + gamma-L-glutamylhydroxamate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlnA | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanohalophilus portucalensis |
| 60 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Methanohalophilus portucalensis |
| 7 | - |
assay at | Archaeoglobus fulgidus |
| 7 | - |
assay at | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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