Application | Comment | Organism |
---|---|---|
drug development | NadE is a antimycobacterial drug target | Mycolicibacterium smegmatis |
drug development | NadE is a antimycobacterial drug target | Mycobacterium tuberculosis |
pharmacology | NadE is a antimycobacterial drug target | Mycolicibacterium smegmatis |
pharmacology | NadE is a antimycobacterial drug target | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockout of gene nadE | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycolicibacterium smegmatis | |
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH3 | Mycolicibacterium smegmatis | - |
AMP + diphosphate + NAD+ | - |
? | |
ATP + deamido-NAD+ + NH3 | Mycobacterium tuberculosis | - |
AMP + diphosphate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | A0A0U0RRS4 | - |
- |
Mycolicibacterium smegmatis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deamido-NAD+ + NH3 | - |
Mycolicibacterium smegmatis | AMP + diphosphate + NAD+ | - |
? | |
ATP + deamido-NAD+ + NH3 | - |
Mycobacterium tuberculosis | AMP + diphosphate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD synthetase | - |
Mycolicibacterium smegmatis |
NAD synthetase | - |
Mycobacterium tuberculosis |
NadE | - |
Mycolicibacterium smegmatis |
NadE | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycolicibacterium smegmatis | |
ATP | - |
Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
malfunction | induced degradation of NadE enzyme shows a strong bactericidal effect which coincided with anticipated changes in relative levels of NaAD intermediate (substrate of NadE) and ultimate depletion of the NAD(H) pool. The disruption of NAD production in the cell via genetic suppression of the essential enzymes (NadD and NadE) involved in the last two steps of NAD biogenesis leads to cell death, even under dormancy conditions. Proteolytic degradation of the enzyme leads to depletion of the NAD cofactor pool, which suppresses metabolic flux through numerous NAD(P)-dependent pathways of central metabolism of carbon and energy production, phenotype, overview | Mycolicibacterium smegmatis |
malfunction | the disruption of NAD production in the cell via genetic suppression of the essential enzymes (NadD and NadE) involved in the last two steps of NAD biogenesis leads to cell death, even under dormancy conditions. Proteolytic degradation of either target enzyme leads to depletion of the NAD cofactor pool, which suppresses metabolic flux through numerous NAD(P)-dependent pathways of central metabolism of carbon and energy production | Mycobacterium tuberculosis |
metabolism | NaMN adenylyltransferase (NadD) and NAD synthetase (NadE) are the key enzymes of NAD biosynthesis | Mycolicibacterium smegmatis |
metabolism | NaMN adenylyltransferase (NadD) and NAD synthetase (NadE) are the key enzymes of NAD biosynthesis | Mycobacterium tuberculosis |