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Literature summary for 6.3.2.1 extracted from

  • Genschel, U.; Powell, C.A.; Abell, C.; Smith, A.G.
    The final step of pantothenate biosynthesis in higher plants: cloning and characterization of pantothenate synthetase from Lotus japonicus and Oryza sativum (rice) (1999), Biochem. J., 341, 669-678.
No PubMed abstract available
Search for more literature for 6.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Oryza sativa
expression Escherichia coli Lotus japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 (R)-pantoate 25°C, pH 8.0 Lotus japonicus
0.44
-
 beta-Alanine 25°C, pH 8.0, at 20 mM pantoate Lotus japonicus
0.98
-
 beta-Alanine 25°C, pH 8.0, at 0.5 mM pantoate Lotus japonicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Lotus japonicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33900
-
 x * 33900, deduced from nucleotide sequence Oryza sativa
34000
-
 2 * 34000, SDS-PAGE Lotus japonicus
72800
-
 gel filtration Lotus japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantoate + beta-alanine Lotus japonicus
-
 AMP + diphosphate + (R)-pantothenate
-
 ?
ATP + (R)-pantoate + beta-alanine Oryza sativa
-
 AMP + diphosphate + (R)-pantothenate
-
 ?

Organism

Organism UniProt Comment Textmining
Lotus japonicus O24035
-
-
Oryza sativa O24210
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant pantothenate snthetase Lotus japonicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.36
-
-
 Lotus japonicus

Storage Stability

Storage Stability Organism
-20°C, no loss of activity Lotus japonicus
4°C, 4 weeks, 25% loss of activity Lotus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate + beta-alanine
-
 Lotus japonicus AMP + diphosphate + (R)-pantothenate
-
 ?
ATP + (R)-pantoate + beta-alanine
-
 Oryza sativa AMP + diphosphate + (R)-pantothenate
-
 ?

Subunits

Subunits Comment Organism
? x * 33900, deduced from nucleotide sequence Oryza sativa
dimer 2 * 34000, SDS-PAGE Lotus japonicus

Synonyms

Synonyms Comment Organism
Pantothenate synthetase
-
 Lotus japonicus
Pantothenate synthetase
-
 Oryza sativa
PS
-
 Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.62
-
 (R)-pantoate 25°C, pH 8.0 Lotus japonicus
6.08
-
 (R)-pantoate 25°C, pH 8.0 Lotus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 in Tris-HCl Lotus japonicus
8
-
 in potassium phosphate Lotus japonicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 50% of maximal activity at pH 7.0, 15% of maximal activity at pH 6.0 and pH 9.0 respectively Lotus japonicus
7.1 9 in Tris-HCl, no activity at pH 7.0, 75% of maximal activity at pH 9.0 Lotus japonicus