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Literature summary for 6.3.2.17 extracted from

  • Bognar, A.L.; Shane, B.
    Bacterial folylpoly(gamma-glutamate) synthase-dihydrofolate synthase (1986), Methods Enzymol., 122, 349-359.
    View publication on PubMed
Search for more literature for 6.3.2.17

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

General Stability

General Stability Organism
K+ and phosphate stabilize Corynebacterium sp.
K+ and phosphate stabilize Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 5,6,7,8-tetrahydropteroyl-Glu
-
 Corynebacterium sp.
0.0023
-
 5,10-methylene-5,6,7,8-tetrahydropteroyl-Glu2
-
 Lacticaseibacillus casei
0.018
-
 ATP
-
 Corynebacterium sp.
0.16
-
 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu L-Glu Corynebacterium sp.
0.423
-
 L-Glu
-
 Lacticaseibacillus casei
5.6
-
 ATP
-
 Lacticaseibacillus casei

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required form maximal activity Escherichia coli
K+ required form maximal activity Corynebacterium sp.
K+ required form maximal activity Lacticaseibacillus casei
K+ maximal activity at 200 mM Escherichia coli
K+ maximal activity at 200 mM Corynebacterium sp.
K+ maximal activity at 200 mM Lacticaseibacillus casei
Mg2+ required Escherichia coli
Mg2+ required Corynebacterium sp.
Mg2+ required Lacticaseibacillus casei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 gel filtration Lacticaseibacillus casei
51000
-
 gel filtration Corynebacterium sp.
53000
-
 1 * 53000, SDS-PAGE Corynebacterium sp.

Organism

Organism UniProt Comment Textmining
Corynebacterium sp.
-
-
-
Escherichia coli
-
-
-
Lacticaseibacillus casei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corynebacterium sp.
-
 Lacticaseibacillus casei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.23
-
-
 Lacticaseibacillus casei
0.57
-
-
 Corynebacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
-
 Lacticaseibacillus casei ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate
-
 Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate ATP in form of MgATP2- Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu + L-glutamate ATP in form of MgATP2- Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-beta-Ala-Glu2
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Corynebacterium sp. ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu n: 1 Lacticaseibacillus casei ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
additional information enzyme also posseses dihydrofolate synthetase activity Escherichia coli ?
-
 ?
additional information enzyme also posseses dihydrofolate synthetase activity Corynebacterium sp. ?
-
 ?
additional information no dihydrofolate synthetase activity Lacticaseibacillus casei ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Escherichia coli
monomer
-
 Lacticaseibacillus casei
monomer 1 * 53000, SDS-PAGE Corynebacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Corynebacterium sp.
9.5
-
-
 Lacticaseibacillus casei