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Literature summary for 6.3.2.17 extracted from

  • Pristupa, Z.B.; Vickers, P.J.; Sephton, G.B.; Scrimgeour, K.G.
    Folylpolyglutamate synthetase from beef liver: assay, stabilization, and characterization (1984), Can. J. Biochem. Cell Biol., 62, 495-506.
    View publication on PubMed
Search for more literature for 6.3.2.17

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol maximal activity at 30-50 mM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 5,6,7,8-tetrahydropteroyl-Glu
-
 Bos taurus
0.01
-
 ATP 5-formyl-5,6,7,8-tetrahydropteroyl-Glu Bos taurus
0.025
-
 aminopterin ATP Bos taurus
0.09
-
 methotrexate
-
 Bos taurus
0.1
-
 methotrexate aminopterin Bos taurus
0.82
-
 L-Glu
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ maximal activity at 10-20 mM Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-20°C, stable for 3 weeks Bos taurus
0°C, in phosphate-mercaptoethanol, pH 7.5, 75% of activity is retained after 10 days, 55% after 3 weeks Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5,6,7,8-tetrahydropteroyl-Glu + L-glutamate
-
 Bos taurus ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
ATP + 5,6,7,8-tetrahydropteroyl-Glun + Glu
-
 Bos taurus ADP + phosphate + 5,6,7,8-tetrahydropteroyl-gamma-Glun+1
-
 ?
ATP + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glun + Glu
-
 Bos taurus ADP + phosphate + 5-formyl-5,6,7,8-tetrahydropteroyl-gamma-Glu2
-
 ?
ATP + aminopterin + Glu most active substrate Bos taurus ADP + phosphate + aminopteryl-Glu
-
 ?
ATP + methotrexate + Glu
-
 Bos taurus ADP + phosphate + methotrexyl-Glu
-
 ?
ATP + pteroyl-Glu + Glu
-
 Bos taurus ADP + phosphate + pteroylmonoglutamyl-Glu
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 glutamylation of methotrexate Bos taurus