Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lacticaseibacillus casei |
Crystallization (Comment) | Organism |
---|---|
enzyme-MgAMPPCP complex is produced by soaking apoFPGS crystals, grown overnight in a solution containing 20% PEG4000, 10 mM AMPPCP, 20 mM MgCl2, 100 mM KCl and 50 mM CAPSO, pH 9.0. The enzyme has a ATP-binding pocket in deep cleft formed between the N and C-domains, the folate-binding site is located in the interdomain cleft, His316, Ser412 and Lys346 are involved in the L-glutamate binding site | Lacticaseibacillus casei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus casei | P15925 | - |
- |
Purification (Comment) | Organism |
---|---|
chromatography on a chitin column | Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate | - |
Lacticaseibacillus casei | ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu | - |
? | |
ATP + 5,6,7,8-tetrahydrofolic acid + L-glutamate | - |
Lacticaseibacillus casei | ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu | - |
? |