Literature summary for 6.3.2.17 extracted from

Wang, P.; Wang, Q.; Yang, Y.; Coward, J.K.; Nzila, A.; Sims, P.F.; Hyde, J.E.
Characterisation of the bifunctional dihydrofolate synthase-folylpolyglutamate synthase from Plasmodium falciparum; a potential novel target for antimalarial antifolate inhibition (2010), Mol. Biochem. Parasitol., 172, 41-51.

Search for more literature for 6.3.2.17

Application

Application	Comment	Organism
medicine	target for antimalarial therapy	Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21 cells	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
2-[[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]phenyl)(hydroxy)phosphoryl]methyl]pentanedioic acid	-	Plasmodium falciparum
2-[[[(3S)-3-[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]benzoyl)amino]-3-carboxypropyl](hydroxy)phosphoryl]methyl]pentanedioic acid	-	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00096	-	5,6,7,8-tetrahydrofolate	-	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 5,6,7,8-tetrahydrofolate + L-glutamate	-	Plasmodium falciparum	ADP + phosphate + 5,6,7,8-tetrahydrofolyl-Glu	-	?
ATP + L-glutamate + tetrahydrofolate-Glu	assay at pH 10, 37°C	Plasmodium falciparum	ADP + phosphate + tetrahydrofolyl-Glu2	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
folypolyglutamate synthase	-	Plasmodium falciparum
FPGS	-	Plasmodium falciparum
PfDHFS-FPGS	bifunctional protein together with dihydrofolate synthase, EC 6.3.2.12	Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Plasmodium falciparum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
10	-	assay at	Plasmodium falciparum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000091	-	2-[[[(3S)-3-[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]benzoyl)amino]-3-carboxypropyl](hydroxy)phosphoryl]methyl]pentanedioic acid	-	Plasmodium falciparum
0.00063	-	2-[[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]phenyl)(hydroxy)phosphoryl]methyl]pentanedioic acid	-	Plasmodium falciparum

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00039	-	-	Plasmodium falciparum	2-[[[(3S)-3-[(4-[[(2-amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino]benzoyl)amino]-3-carboxypropyl](hydroxy)phosphoryl]methyl]pentanedioic acid

Expression

Organism	Comment	Expression
Plasmodium falciparum	expressed as single bifunctional protein with dihydrofolate synthase, EC 6.3.2.12	additional information

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Release 2023.1 (January 2023)