Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by DTT | Thermosynechococcus vestitus |
Cloned (Comment) | Organism |
---|---|
gene cphA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional overexpression of His-tagged Tlr2170 in Escherichia coli BL21(DE3) | Thermosynechococcus vestitus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activates | Thermosynechococcus vestitus | |
Mg2+ | Mg2+ or Mn2+ are required | Thermosynechococcus vestitus | |
Mn2+ | Mg2+ or Mn2+ are required | Thermosynechococcus vestitus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
4 * 100000, SDS-PAGE | Thermosynechococcus vestitus |
400000 | - |
the molecular mass of cyanophycin increases with increasing reaction temperature | Thermosynechococcus vestitus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | Thermosynechococcus vestitus | - |
ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | - |
? | |
additional information | Thermosynechococcus vestitus | CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | - |
gene cphA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme by nickel affinity chromatography and gel filtration | Thermosynechococcus vestitus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | - |
Thermosynechococcus vestitus | ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | - |
? | |
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | primer and product analysis using recombinantly expressed Tlr2170 protein, overview | Thermosynechococcus vestitus | ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | - |
? | |
additional information | CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview | Thermosynechococcus vestitus | ? | - |
? | |
additional information | the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29. Recombinant Tlr2170 protein catalyzes in vitro cyanophycin synthesis in the absence of a primer. The Tlr2170 protein shows strict substrate specificity toward L-Asp and L-Arg | Thermosynechococcus vestitus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 100000, SDS-PAGE | Thermosynechococcus vestitus |
Synonyms | Comment | Organism |
---|---|---|
CphA | - |
Thermosynechococcus vestitus |
cyanophycin synthetase | - |
Thermosynechococcus vestitus |
More | cf. EC 6.3.2.29 | Thermosynechococcus vestitus |
Tlr2170 protein | - |
Thermosynechococcus vestitus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Thermosynechococcus vestitus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | - |
Thermosynechococcus vestitus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant Tlr2170 protein retains 80% activity after a 60 min incubation at 50°C | Thermosynechococcus vestitus |
70 | - |
purified recombinant Tlr2170, inactivation | Thermosynechococcus vestitus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Thermosynechococcus vestitus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermosynechococcus vestitus |