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Literature summary for 6.3.2.30 extracted from

  • Hai, T.; Lee, J.S.; Kim, T.J.; Suh, J.W.
    The role of the C-terminal region of cyanophycin synthetase from Nostoc ellipsosporum NE1 in its enzymatic activity and thermostability: A key function of Glu(856) (2008), Biochim. Biophys. Acta, 1794, 42-49.
    View publication on PubMed
Search for more literature for 6.3.2.30

Cloned(Commentary)

Cloned (Comment) Organism
gene cphA, subcloning and expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3) Nostoc ellipsosporum

Protein Variants

Protein Variants Comment Organism
E856A site-directed mutagenesis, inactive mutant Nostoc ellipsosporum
E856V site-directed mutagenesis, inactive mutant Nostoc ellipsosporum
additional information construction of truncation mutant CphANE1-C45, which lacks up to 45 amino acids at its C-terminus, the mutant retains full enzymatic activity and produced polymers, however, the removal of one additional amino acid, Glu856, results in complete inactivation of CphANE1-C46, phenotype, overview. Removal of the sequence Leu867-Leu870 results in dramatically decreased thermostability Nostoc ellipsosporum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Nostoc ellipsosporum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
210000
-
 gel filtration, recombinant wild-type enzyme Nostoc ellipsosporum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Nostoc ellipsosporum
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Nostoc ellipsosporum NE1
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Nostoc ellipsosporum
-
-
-
Nostoc ellipsosporum NE1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Nostoc ellipsosporum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activities of recombinant truncation mutant enzymes, overview Nostoc ellipsosporum
17.5
-
 purified recombinant wild-type enzyme at 50°C Nostoc ellipsosporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Nostoc ellipsosporum ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg role of the C-terminal region of CphANE1, Glu856 is critical for CphANE1 catalytic activity, overview Nostoc ellipsosporum ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Nostoc ellipsosporum NE1 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg role of the C-terminal region of CphANE1, Glu856 is critical for CphANE1 catalytic activity, overview Nostoc ellipsosporum NE1 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
additional information the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29 Nostoc ellipsosporum ?
-
 ?
additional information the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29 Nostoc ellipsosporum NE1 ?
-
 ?

Subunits

Subunits Comment Organism
dimer wild-type and truncation mutant CphANE1-C45 Nostoc ellipsosporum

Synonyms

Synonyms Comment Organism
CphA
-
 Nostoc ellipsosporum
cyanophycin synthetase
-
 Nostoc ellipsosporum
More cf. EC 6.3.2.29 Nostoc ellipsosporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Nostoc ellipsosporum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the predicted alpha-helical region that ranges from Val858 to Leu870 is important for the thermostability of the enzyme Nostoc ellipsosporum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Nostoc ellipsosporum