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Literature summary for 6.3.2.8 extracted from

  • Liger, D.; Masson, A.; Blanot, D.; van Heijenoort, J.; Parquet, C.
    Study of the overproduced uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli (1996), Microb. Drug Resist., 2, 25-27.
    View publication on PubMed
Search for more literature for 6.3.2.8

General Stability

General Stability Organism
stability is strictly dependent on the presence of 2-mercaptoethanol Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
3-chloro-L-Ala
-
 Escherichia coli
3-Cyano-L-Ala
-
 Escherichia coli
3-Fluoro-L-Ala
-
 Escherichia coli
NEM partial protection by ATP Escherichia coli
p-hydroxymercuribenzoate partial protection by ATP Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 L-Ala
-
 Escherichia coli
0.1
-
 UDP-N-acetylmuramate
-
 Escherichia coli
0.45
-
 ATP
-
 Escherichia coli
0.85
-
 Ser
-
 Escherichia coli
2.5
-
 Gly
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53630
-
 mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + UDP-N-acetylmuramate + L-Ala Escherichia coli adds the first amino acid to the sugar moiety of the peptidoglycan precursor, catalyzing one essential step in cell wall biosynthesis ?
-
 ?
ATP + UDP-N-acetylmuramate + L-Ala Escherichia coli formation of a acylphosphate intermediate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 JM83(pAM1005), overproduced in a strain harboring a recombinant plasmid pAM1005 with murC gen under the control of the inducible promoter trc
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetylmuramate + DL-Ser
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser
-
 ?
ATP + UDP-N-acetylmuramate + Gly
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
 ?
ATP + UDP-N-acetylmuramate + L-Ala r Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?
ATP + UDP-N-acetylmuramate + L-Ala adds the first amino acid to the sugar moiety of the peptidoglycan precursor, catalyzing one essential step in cell wall biosynthesis Escherichia coli ?
-
 ?
ATP + UDP-N-acetylmuramate + L-Ala formation of a acylphosphate intermediate Escherichia coli ?
-
 ?
ATP + UDP-N-acetylmuramate + L-serine
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli