Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is usable in theanine formation by coupling with the alcoholic fermentation system of bakers yeast, Saccharomyces cerevisiae | Methylovorus mays |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain AD494(DE3) | Methylovorus mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | ethylamine inhibits yeast sugar fermentation and recombinant theanine production in vivo in a coupled theanine-foorming reaction with Saccharomyces cerevisiae, which can be overcome by potassium phosphate addition, overview | Methylovorus mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requred, slightly stimulating | Methylovorus mays | |
Mn2+ | stimulates | Methylovorus mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + ethylamine | Methylovorus mays | a step in theanine biosynthesis | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
ATP + L-glutamate + ethylamine | Methylovorus mays No. 9 | a step in theanine biosynthesis | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
ATP + L-glutamate + methylamine | Methylovorus mays | - |
ADP + phosphate + N5-methyl-L-glutamine | - |
? | |
ATP + L-glutamate + methylamine | Methylovorus mays No. 9 | - |
ADP + phosphate + N5-methyl-L-glutamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylovorus mays | - |
- |
- |
Methylovorus mays No. 9 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain AD494(DE3) by ammonium sulfate fractionation and anion exchange chromatography | Methylovorus mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + ethylamine | - |
Methylovorus mays | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
ATP + L-glutamate + ethylamine | a step in theanine biosynthesis | Methylovorus mays | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
ATP + L-glutamate + ethylamine | - |
Methylovorus mays No. 9 | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
ATP + L-glutamate + ethylamine | a step in theanine biosynthesis | Methylovorus mays No. 9 | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
ATP + L-glutamate + methylamine | - |
Methylovorus mays | ADP + phosphate + N5-methyl-L-glutamine | - |
? | |
ATP + L-glutamate + methylamine | - |
Methylovorus mays No. 9 | ADP + phosphate + N5-methyl-L-glutamine | - |
? | |
additional information | ammonia is a poor substrate | Methylovorus mays | ? | - |
? | |
additional information | ammonia is a poor substrate | Methylovorus mays No. 9 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-Glutamylmethylamide synthetase | - |
Methylovorus mays |
GMAS | - |
Methylovorus mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Methylovorus mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Methylovorus mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methylovorus mays |